Isothermal Amplification Reagents
Isothermal Amplification Reagents
Invitrogen™ L.b. Cas12a Nuclease
L.b. Cas12a Nuclease for simple, quick and specific analysis of your samples.
Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.
Thermo Scientific™ phi29 DNA Polymerase (10 U/μL)
Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification.
Thermo Scientific™ Bsm DNA Polymerase, large fragment (8 U/μL)
Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities.
Invitrogen™ SuperScript™ IV RT-LAMP Master Mix
Invitrogen SuperScript IV RT-LAMP Master Mix is a ready-to-use solution optimized for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions.
Invitrogen™ Lyo-ready Bst DNA Polymerase
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors.
Thermo Scientific™ Reaction Buffer for phi29 DNA Polymerase (10X)
Thermo Scientific 10X Buffer for phi29 DNA Polymerase is the optimal buffer recommended for use with highly processive phi29 DNA polymerase.
Thermo Scientific™ EquiPhi29™ DNA Amplification Kit
The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).
Thermo Scientific™ EquiPhi29™ DNA Polymerase
Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution.
Thermo Scientific™ EquiPhi29™ DNA Polymerase Reaction Buffer (10X)
This reaction buffer is for use with EquiPhi29 DNA Polymerase. Buffer is included with purchase of the polymerase, but additional buffer is made available here to help accommodate experimental needs.
New England Biolabs, Inc. phi29-XT RCA DNA Ampification Kit - 500 reactions
The phi29-XT RCA DNA Amplification Kit includes everything needed for sensitive and robust rolling circle amplification (RCA), an isothermal amplification approach to continuously amplify circular DNA by generating long, repetitive copies of the circular sequence. RCA enables several downstream applications, including DNA sequencing, cell-free protein expression, viral DNA enrichment, plasmid amplification, and padlock probe-based assays. The kit features phi29-XT DNA Polymerase, an engineered polymerase with improved thermostability, sensitivity, and capable of generating higher yield in a shorter reaction time than wild-type phi29 DNA Polymerase. Also included are exonuclease-resistant random primers (containing phosphorothioate bonds) to universally amplify any circular DNA sequence and dNTPs. Input material can be a purified plasmid, liquid media culture, a cultured colony from an agar plate, or a glycerol stock.
BIOSEARCH TECHNOLOGIES INC LavaLAMP DNA Master Mix with Dye, 200 ReactionsSensitive and Fast DNA LAMP in an easy-to-use Master Mix format.
Sensitive and Fast DNA LAMP in an easy-to-use Master Mix format.Loop-mediated isothermal amplification (LAMP): Facilitates running amplification reactions in difficult test environments, enabling use outside of a high-tech laboratory.Master Mix Format: Simplifies reaction setup and minimizes optimization required to produce the best assay results.High Thermal Stability: Highly thermostable enzyme (up to 90 C for 5 min) enables preheating of reactions containing purified target DNA which, depending on the target, may increase assay sensitivity and decrease time to results.Elevated LAMP Reaction Temperature (68-74 C):Improves primer specificity and reduces background amplification depending on the DNA target.Both LavaLAMP DNA Master Mix kits contain: LavaLAMP DNA Master Mix, DNA Positive Control LAMP Primer Mix, and DNA Positive Control. The LavaLAMP DNA Master Mix with Dye also contains Green Fluorescent Dye for fluorescent detection of amplified DNA.
New England Biolabs, Inc. phi29-XT RCA DNA Amplification Kits - 100 reactions
The phi29-XT RCA DNA Amplification Kit includes everything needed for sensitive and robust rolling circle amplification (RCA), an isothermal amplification approach to continuously amplify circular DNA by generating long, repetitive copies of the circular sequence. RCA enables several downstream applications, including DNA sequencing, cell-free protein expression, viral DNA enrichment, plasmid amplification, and padlock probe-based assays. The kit features phi29-XT DNA Polymerase, an engineered polymerase with improved thermostability, sensitivity, and capable of generating higher yield in a shorter reaction time than wild-type phi29 DNA Polymerase. Also included are exonuclease-resistant random primers (containing phosphorothioate bonds) to universally amplify any circular DNA sequence and dNTPs. Input material can be a purified plasmid, liquid media culture, a cultured colony from an agar plate, or a glycerol stock.
