Restriction Enzymes
Restriction Enzymes
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Filtered Search Results
Thermo Scientific™ DpnI (10 U/μL)
Cut at Gm6A^TC sites with DpnI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: MalI).
Concentration | 10 U/μL |
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Thermo Scientific™ FspBI (BfaI) (10 U/μL)
The FspBI (BfaI) restriction enzyme recognizes C^TAG sites and cuts best at 37°C in Tango buffer (Isoschizomers: BfaI, MaeI, XspI).
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | FspBI (BfaI) |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer Tango |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ HhaI (10 U/μL)
Cut at GCG^C sites with HhaI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AspLEI, BstHHI, CfoI, HinP1I, HspAI).
Thermo Scientific™ AlfI
Cut at ^(10/12)GCA(N)6TGC(12/10)^ sites with AlfI restriction enzyme, which performs best at 37°C in R (+SAM) buffer.
Thermo Scientific™ EcoRII (10 U/μL)
Cut at ^CCWGG sites with EcoRII restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI).
Thermo Scientific™ LguI (SapI) (5 U/μL)
The LguI (SapI) restriction enzyme recognizes GCTCTTC(1/4)^ sites and cuts best at 37°C in Tango buffer (Isoschizomers: BspQI, PciSI, SapI).
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | LguI (SapI) |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Scarless Cloning |
Compatible Buffer | 10X Buffer Tango |
Type IIS RE | Yes |
Optimal Reaction Temperature | 37°C |
Concentration | 5 U/μL |
Thermo Scientific™ FastDigest Eco47III
Cut at AGC^GCT sites with Thermo Scientific™ FastDigest™ AfeI (Eco47III), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ FastDigest Eco91I
Cut at G^GTNACC sites with Thermo Scientific™ FastDigest™ Eco91I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ FastDigest Eco130I
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest NcoI
The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Nco I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
InactivationTemperature | 65°C |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest AluI
Cut at AG^CT sites with Thermo Scientific™ FastDigest™ AluI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AluBI).
Thermo Scientific™ FastDigest Eco52I
Cut at C^GGCCG sites with Thermo Scientific™ FastDigest™ EagI (Eco52I), which performs best universal FastDigest Buffer (Isoschizomers: BseX3I, BstZI, EclXI).
Thermo Scientific™ FastDigest NotI
The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI).
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Not I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
InactivationTemperature | 80°C |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest SalI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest TaqI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.