DNA Vectors
DNA Vectors
Invitrogen™ S.c. EasyComp™ Transformation Kit
Designed for rapid preparation of transformation-competent Saccharomyces cerevisiae cells
Invitrogen™ Pichia EasyComp™ Transformation Kit
Designed for rapid preparation and transformation of competent Pichia pastoris cells
MilliporeSigma™ Novagen™ Perfectly Blunt™ pSTBlue-1 Giga Cloning Kit
For increased transformation efficiency
Invitrogen™ Champion™ pET102 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli
Protein of interest can reach levels greater than 50 percent of total cellular protein in E. coli during expression
Invitrogen™ Champion™ pET100 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli
Yields protein of interest at levels greater than 50 percent of total cellular protein in E. coli
Invitrogen™ Champion™ pET151 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli
Protein of interest can reach levels greater than 50 percent of total cellular protein during expression
Promega pAdVAntage™ Vector
Co-transfection of mammalian cells with the pAdVAntage Vector enhances transient protein expression in a variety of cell types by increasing translation initiation.
Invitrogen™ Champion™ pET101 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli
Yields protein of interest at levels greater than 50 percent of total cellular protein in E. coli
Invitrogen™ GS115, Pichia pastoris Yeast Strain
Optimize expression and recovery of protein of interest
Invitrogen™ X-33, Pichia pastoris Yeast Strain
Optimize expression and recovery of protein of interest
Clontech Labs 3P pPUR Vector | 25ug
pPUR confers puromycin resistance to mammalian cells and can be used to select for stably transformed cell lines.
Boca Scientific Inc pPICHOLI Shuttle Vector System
The pPICHOLI vectors are designed for heterologous gene expression in the yeast P. pastoris and in the prokaryote E. coli. The vectors contain an inducible (yeast) alcohol oxidase (AOX) promoter and an E. coli T7 promoter. The vectors also contain sequences allowing autonomous replication both in P. pastoris and E. coli. Due to these components, vector linearization is no longer required and small amounts of DNA are sufficient to successfully transform P. pastoris. The integrated PARS sequence enables simple recovery of plasmids from yeast. Time-consuming subcloning into several expression vectors, including testing for a successful gene expression, is no longer necessary. A multiple cloning site enables convenient ligation of DNA fragments into the vectors.
IBA LifeSciences pASK-IBA2C vector for E. coli with C-terminal Strep-tagII; 5 µg
pASK-IBA2C is a classic cloning vector devised for E. coli expression of recombinant proteins with the Strep-tagII fused to the C-terminus. It carries the chloramphenicol resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, a multiple cloning site with recognition sites for several common restriction enzymes (e.g. EcoRI, SmaI, BamHI, SalI, PstI), the inducible tetracycline promoter/operator for the regulated expression of proteins, and the ompA signal for periplasmic secretion of the recombinant protein. The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During secretion into the periplasmic space the ompA signal sequence will be cleaved off.
Clontech Labs 3P T-Vector pMD™19 (Simple), 1ug
T-Vector pMD19 (Simple) is a linearized vector with a single 3'-terminal thymidine at both ends. The T-overhang ends at the cloning site improve the efficiency of ligation of PCR products which contain A-overhangs at 3' -ends. The multiple cloning sites in the lac Z gene sequence have been deleted from this vector, but its β-galactosidase activity is not disrupted. Therefore, transformant colonies containing recombinant plasmids can be identified by blue/white screening. Due to lack of multiple cloning sites in the vector, the inserted PCR product cannot be recut using restriction enzymes. When the insert DNA fragment is recut from the vector, the PCR primers should be used to add a restriction site to the 5'-end for restriction enzyme digestion and subcloning. T-Vector pMD19 (Simple) is supplied with a Control Insert (500 bp) DNA for positive control reactions.