Molecular Biology Reagents and Kits
Molecular Biology Reagents and Kits
Invitrogen™ Pichia EasyComp™ Transformation Kit
Designed for rapid preparation and transformation of competent Pichia pastoris cells
Invitrogen™ S.c. EasyComp™ Transformation Kit
Designed for rapid preparation of transformation-competent Saccharomyces cerevisiae cells
MilliporeSigma™ Novagen™ Perfectly Blunt™ pSTBlue-1 Giga Cloning Kit
For increased transformation efficiency
Invitrogen™ Champion™ pET102 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli
Protein of interest can reach levels greater than 50 percent of total cellular protein in E. coli during expression
Invitrogen™ Champion™ pET151 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli
Protein of interest can reach levels greater than 50 percent of total cellular protein during expression
Invitrogen™ Champion™ pET100 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli
Yields protein of interest at levels greater than 50 percent of total cellular protein in E. coli
Promega pAdVAntage™ Vector
Co-transfection of mammalian cells with the pAdVAntage Vector enhances transient protein expression in a variety of cell types by increasing translation initiation.
Invitrogen™ Champion™ pET101 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli
Yields protein of interest at levels greater than 50 percent of total cellular protein in E. coli
Invitrogen™ GS115, Pichia pastoris Yeast Strain
Optimize expression and recovery of protein of interest
Invitrogen™ X-33, Pichia pastoris Yeast Strain
Optimize expression and recovery of protein of interest
Clontech Labs 3P pPUR Vector | 25ug
pPUR confers puromycin resistance to mammalian cells and can be used to select for stably transformed cell lines.
![Zymo Research Corporation Zymoprep™ Yeast Plasmid Miniprep II (50 Preps) w/ Zymo-Spin™ I Columns (Uncapped)[Includes E1004 x 1: Zymolyase (1000 Units Lyophilized) w/ Storage Buffer (500µl)]](https://assets.fishersci.com/TFS-Assets/CCG/product-images/OS-D2001ZYMOPREP-YEAST-PLASMID-MINIPREP-I-KIT1.JPG-250.jpg)
Zymo Research Corporation Zymoprep™ Yeast Plasmid Miniprep II (50 Preps) w/ Zymo-Spin™ I Columns (Uncapped)[Includes E1004 x 1: Zymolyase (1000 Units Lyophilized) w/ Storage Buffer (500µl)]
The Zymoprep™ Yeast Plasmid Miniprep II provides all the necessary reagents for plasmid isolation from S. cerevisiae, C. albicans and S. pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The procedure is simple and efficient, and there is no need for glass beads or phenol. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and hard to isolate plasmids. Eluted plasmid DNA can be used directly for E. Coli transformation, PCR, and Southern blot analysis.
Agilent Technologies IPTG
IPTG is used in combination with X-gal to detect beta-galactosidase activity with a white/blue color assay and also for screening of transformed colonies.
Biochain Institute Inc Express Cloning Checker Kit II, 100 rxn/PK
BioChain's Express Cloning Checker Kits provide highly efficient methods of identifying recombinant colonies after transformation eliminating time-consuming plasmid preparation. In the large-scale screen method, the partial colonies of E. coli bacteria can be directly transfered from the transformation plates into the solutions of the kits and immediately run on an agarose gel electrophoresis. The results will be known within 1-2 hours instead of the 2 days required in a conventional method of labor-intensive plasmid DNA miniprep or colony hybridization.
Biochain Institute Inc Express Cloning Checker Kit II: Blue Solution, 1 ml/PK
BioChain's Express Cloning Checker Kits provide highly efficient methods of identifying recombinant colonies after transformation eliminating time-consuming plasmid preparation. In the large-scale screen method, the partial colonies of E. coli bacteria can be directly transfered from the transformation plates into the solutions of the kits and immediately run on an agarose gel electrophoresis. The results will be known within 1-2 hours instead of the 2 days required in a conventional method of labor-intensive plasmid DNA miniprep or colony hybridization.