The materials contained in this Salmonella Mutagenicity Assay Kit include virtually all of the supplies necessary for the conduct of the “Ames Assay” as described by Maron & Ames (Maron, D. M. and B. N. Ames, Revised methods for the Salmonella mutagenicity test, Mutation Research, 113: 173-215, 1983) and Mortelmans & Zeiger (Mortelmans, K. and E. Zeiger, The Ames Salmonella/microsome mutagenicity assay, Mutation Research, 455: 29-60, 2000). We strongly recommend that you carefully read one of these papers and OECD guideline 471 before you attempt to perform the assay. This kit contains four tester strains; TA1535, TA1537, TA98, and TA100. Tester strains TA98 and TA100 carry a plasmid (pKM101); TA1535 and TA1537 contain no plasmids. The MOLTOX 31-100.2 kit includes sufficient components for testing 1 unknown sample, in triplicate analysis of 5 concentrations, positive and negative controls, with and without S9.