Restriction Enzyme Buffers
Restriction Enzyme Buffers
Thermo Scientific™ FastDigest Green Buffer (10X)
Proprietary digestion buffer which supports 100% activity of all FastDigest restriction enzymes.
Invitrogen™ Anza™ Buffer Set
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
New England Biolabs, Inc. rCutSmart™ Buffer - 5 mls
New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. These buffers contain Recombinant Albumin (NEB #B9200). Over 215 restriction enzymes are 100% active in rCutSmart Buffer, making it significantly easier to set up your double digest reactions. Since rCutSmart Buffer includes Recombinant Albumin, there are also fewer tubes and pipetting steps to worry about. Additionally, many DNA modifying enzymes are 100% active in rCutSmart Buffer, eliminating the need for subsequent purification.
Zymo Research Corporation 2X Digestion Buffer, 20 mL
Proteinase K digestion buffer for use with Zymo Research DNA and RNA extraction kits. Sold separately or as part of a complete kit.
Zymo Research Corporation 2X Digestion Buffer, 80 mL
Proteinase K digestion buffer for use with Zymo Research DNA and RNA extraction kits. Sold separately or as part of a complete kit.
New England Biolabs, Inc. NEBuffer Set (EcoRI, DpnII) – 1.25 ml of each
New England Biolabs provides 10X NEBuffer with each restriction endonuclease to assure optimal (100%) activity. Set contains two 1.25ml vials of each unique NEBuffer for the enzymes EcoRI and DpnII. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer. 1X NEBuffer EcoRI 100 mM Tris-HCl 50 mM NaCl 10 mM MgCl2 0.025% Triton X-100 (pH 7.5 @ 25C) 1X NEBuffer DpnII 50 mM Bis-Tris-HCl 100 mM NaCl 10 mM MgCl2 1 mM DTT (pH 6 @ 25C)
Zymo Research Corporation 2X Digestion Buffer, 5 mL
Proteinase K digestion buffer for use with Zymo Research DNA and RNA extraction kits. Sold separately or as part of a complete kit.
Edvotek™ Reagents for Electrophoresis: Restriction Enzyme Reaction Buffer
For restriction enzymes.