Nucleic Acid Introduction Reagents
Nucleic Acid Introduction Reagents
Thermo Scientific™ TransformAid Bacterial Transformation Kit
Kit for quick and convenient preparation of competent E.coli cells for guaranted transformation efficiencies of more than 107 transformants per μg of plasmid DNA.
MP Biomedicals™ EZ-Yeast™ Transformation Kit
Designed for high-throughput transformation with efficiencies up to 103 transformants per μg plasmid DNA. Perfect for a quick analysis of potentially positive two-hybrid colonies, the EZ-Yeast™ Transformation Kit does not require fully competent cells prior to transformation.
MP Biomedicals™ Alkali-Cation™ Yeast Transformation Kit
Provides all of the reagents required to perform a quick and easy transformation. Alkali-Cation™ Yeast Transformation Kit is easy to use, robust, and its scalability makes it great for the high-efficiency transformation needed in two hours.
Invitrogen™ MAX Efficiency™ Transformation Reagent for Algae
A buffer that enhances transformation efficiency for multiple strains of Chlamydomonas species
Edvotek™ Reagents For Bacterial Transformation: X-gal
For rapid preparation of bacterial cultures for transformation.
Gibco™ CTS™ Xenon™ Lower Conductivity Electroporation Buffer
Gibco CTS Xenon Lower Conductivity Electroporation Buffer is designed specifically for use with the Xenon Electroporation System.
MilliporeSigma™ Novagen™ ColiRollers™ Plating Beads
Used for plating bacteria for transformation molecular cloning applications.
Bioworld Z-competent transformation kit, 300 Prep(s)
Z-Competent E.coli Transformation KitThe Z-Competent E. coli Transformation Kit is a simple, highly efficient method for preparing cells for transformation. No heat shock or any other manipulation is required. Simply grow cells in SOB medium, wash and resuspend the cells in the Competent Buffer, then the cells are ready to receive DNA. Routinely produces 2 x 108 to 1 x 109 transformants per 1µg supercoiled pUC19 DNA. Competent cells can be used immediately or stored for later use. 300 transformation preparations
Research Products International Corp Frozen-EZ Yeast Transformation II Kit, 120 Rxns
The Frozen-EZ Yeast Transformation II Kit is designed to make yeast transformations and library screening easier and more efficient than currently available methods. The yeast cells prepared with the Frozen-EZ Yeast Transformation II Kit can be used immediately for transformation or can be stored (i.e., ≤ -70 C) for use at a later time. Yeast prepared with this kit can be transformed with both circular and linear DNAs. Also, the Frozen-EZ Yeast Transformation II Kit can be used with other fungi including C. albicans, S. pombe, and P. pastoris.
Yeast cells with high transformation efficiencies can be prepared in under 10 minutes.
Simple method for transforming yeast with single or multiple plasmids in less than 1 hour.
No carrier DNA required.
Research Products International Corp Mix & Go! E.coli Transformation Buffer Set, up to 60 ml
Mix & Go! E.coli Transformation Buffer Set, up to 60 ml
Zymo Research Corporation Frozen-EZ Yeast Transformation II™ Kit (120 Rxns)
Version II further improves the efficiency and reliability ofits predecessor. The competent yeast cells prepared with these reagents can be used immediately for transformation experiments or can be stored frozen at or below -70C for direct use in the future. This method is suitable for both circular and linear plasmid transformations.
Zymo Research Corporation Mix & Go E. coli Transformation Kit & Buffer Set, up to 60 ml
The methods eliminate the requirement of heat-shocking and related procedures. Instead, transformation can be performed by adding DNA to prepared Mix & Go cells and the mixture spread directly to a culture plate. Transformation efficiencies typically range from 108–109 transformants/µg of pUC19 DNA but can vary depending on the strain of E. coli. Most E. coli strains respond well to the Mix & Go preparation method and demonstrate fast transformation kinetics.The procedures are easy. Simply culture the E. coli strain of your choice in ZymoBroth™ medium (or SOB), wash and then resuspend the cells in the provided uniquely formulated buffers. The cells are now ready for transformation.
Cell Biolabs Inc CytoSelect™ 96-Well Cell Transformation Assay Kit (Cell Recovery Compatible, Colorimetric), 96 assays
The traditional soft agar colony formation assay monitors anchorage-independent cell growth by measuring proliferation in a semisolid culture media in tissue culture dishes after 3-4 weeks, followed by manual counting of colonies. This method is cumbersome and not amenable to high throughput, and the manual counting of colonies is subjective because with varying colony sizes, it’s difficult to determine meaningful results. The CytoSelect™ 96-well Cell Transformation Assay (Cell Recovery Compatible) eliminates subjective manual counting of colonies with a much shorter assay time. Cells are incubated only 6-8 days in a semisolid agar media before being solubilized, lysed and detected by the provided colorimetric dye in a standard plate reader, making it a more quantitative and higher-throughput method. Additionally, the proprietary medium makes it possible to recover viable and intact cells for further downstream analysis. Each kit is sufficient for 96 tests in a microtiter plate.
Promega BL21(DE3)pLysS Competent Cells
BL21(DE3)pLysS Competent Cells allow high-efficiency protein expression of any gene that is under the control of a T7 promoter and has a ribosome binding site.