DNA Cloning and Mapping and Mutagenesis
DNA Cloning and Mapping and Mutagenesis
MilliporeSigma™ Novagen™ Clonables™, Ligation/Transformation Kit
Supports ligation of any type of DNA cohesive or blunt ends.
Invitrogen™ GeneArt™ pYES1L Vector with Sapphire™ Technology
A ready-to-use, 9.3 Kb linearized BAC/YAC shuttle vector, used for the assembly of DNA fragments with the GeneArt™ High-Order Genetic Assembly System
Agilent Technologies XL1-Blue Competent Cells. 5 x 0.2 mL. For routine cloning, ≥ 1 x 108 transformants/µg
XL1-Blue Competent Cells, Strain of choice for preparation of high-quality plasmid DNA, Single-strand rescue of phagemid DNA, Contains an antibiotic-resistant F' episome, Available in a wide variety of transformation efficiencies
Promega Single-Step (KRX) Competent Cells
The Single Step (KRX) Competent Cells are designed for efficient transformation and tightly controlled protein expression with one strain.
Agilent Technologies SOLOPACK GOLD SUPERCOMP CELLS
SoloPack Gold Cells for Transformation in the Tube,convenient transformation in the tube, Now even easier, no BME required, robust growth produces large colonies.
Zymo Research Corporation Mix & Go! Competent Cells - DH5 Alpha, 10 x 100 µl
Mix & Go E. coli cells are made chemically competent by a unique method that completely eliminates the need for heat shock and related procedures. For transformation, DNA can be added directly to Mix & Go cells and the mixture spread directly to a culture plate. Transformation efficiencies typically range from 108-109 transformants/µg of pUC19 DNA , which make the cells optimal for cloning, sub-cloning, library construction, etc. Premade Mix & Go cells are supplied as a pack of 10 convenient 100 µl/tube aliquots or in a 96-well format (12 x 8 - tube strips) of 50 µl/tube.
System Biosciences LLC Cold Fusion Cloning Kit with Competent Cells, 50 Reactions
Fast, easy, and efficient, the phosphatase-free, ligation-free Cold Fusion Cloning Kit will take you to transformation-ready DNA in 20 minutes and a single step.
Agilent Technologies BL21-GOLD(DE3)PLYSS C.CELLS
BL21-Gold (DE3)pLysS Competent Cells Provide high-level protein expression and Allow direct cloning of many expression constructs. Hte phenotype increases transformation efficiency to 1 x 108 of supercoiled DNA. Used for toxic proteins.
BIOSEARCH TECHNOLOGIES INC E. cloni EXPRESS BL21(DE3) Electrocompetent Cells, 12 RxnsGet better colony counts for routine protein expression with any E. coli T7 expression vector, with this high-efficiency BL21(DE3) strain.
LucigensE. cloniEXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors preparations of BL21 cells, which is essential for construction of complex expression libraries. The unprecedented transformation efficiency ofE. cloniEXPRESS Electrocompetent Cells (>5 10^9cfu/g) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment. E. cloni EXPRESS cells are also available as chemically competent cells. With a transformation efficiency of >1 10^7 cfu/g and very economical prices, EXPRESS Chemically Competent Cells offer superior value for everyday protein expression work.
New England Biolabs, Inc. Q5 Site-Directed Mutagenesis Kit – 10 reactions
The Q5 Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid (5 minutes), room temperature circularization and template removal. Transformation into high-efficiency chemically-competent E. coli, ensures robust results with plasmids up to at least 20 kb in length.
New England Biolabs, Inc. Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) – 10 reactions
The Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours). The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid (5 minutes), room temperature circularization and template removal. Transformation into high-efficiency chemically-competent E. coli, not supplied, ensures robust results with plasmids up to at least 20 kb in length. Kit is also available with competent cells .
New England Biolabs, Inc. NEBuilder HiFi DNA Assembly Master Mix – 50 reactions
NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. It allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. Used to assemble either ss oligonucleotides or different sizes of DNA frags with varied overlaps. Has utility in synbio community, as well as those interested in one-step cloning of multiple fragments due to its ease of use, flexibility and simple MM format. The rxn includes different enzymes that work together in the same buffer: The exonuclease creates single-stranded 3' overhangs that facilitate the annealing of fragments that share complementarity at one end (the overlap region): a polymerase fills in gaps within each annealed fragment; DNA ligase seals nicks in the assembled DNA. End result is a ds fully sealed DNA molecule that can serve as template for PCR, RCA or a variety of other molecular biology applications, including direct transformation of E coli.
New England Biolabs, Inc. NEBuilder HiFi DNA Assembly Master Mix – 10 reactions
NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. It allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. Used to assemble either ss oligonucleotides or different sizes of DNA frags with varied overlaps. Has utility in synbio community, as well as those interested in one-step cloning of multiple fragments due to its ease of use, flexibility and simple MM format. The rxn includes different enzymes that work together in the same buffer: The exonuclease creates single-stranded 3' overhangs that facilitate the annealing of fragments that share complementarity at one end (the overlap region): a polymerase fills in gaps within each annealed fragment; DNA ligase seals nicks in the assembled DNA. End result is a ds fully sealed DNA molecule that can serve as template for PCR, RCA or a variety of other molecular biology applications, including direct transformation of E coli.