DNA Cloning and Mapping and Mutagenesis
DNA Cloning and Mapping and Mutagenesis
Thermo Scientific™ CloneJET™ PCR Cloning Kit with DH10B Competent Cells
An advanced positive-selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Any blunt or sticky-end DNA fragment can be cloned.
Promega Single-Step (KRX) Competent Cells
The Single Step (KRX) Competent Cells are designed for efficient transformation and tightly controlled protein expression with one strain.
BIOSEARCH TECHNOLOGIES INC CopyCutter EPI400 Chemically Competent E. coli, 10 Rxns
CopyCutter™ EPI400™ E. coli cells will significantly lower the copy number of a wide variety of common vectors to enable cloning of unstable DNA sequences and those expressing toxic proteins
BIOSEARCH TECHNOLOGIES INC E. cloni EXPRESS BL21(DE3) Electrocompetent Cells, 12 RxnsGet better colony counts for routine protein expression with any E. coli T7 expression vector, with this high-efficiency BL21(DE3) strain.
LucigensE. cloniEXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors preparations of BL21 cells, which is essential for construction of complex expression libraries. The unprecedented transformation efficiency ofE. cloniEXPRESS Electrocompetent Cells (>5 10^9cfu/g) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment. E. cloni EXPRESS cells are also available as chemically competent cells. With a transformation efficiency of >1 10^7 cfu/g and very economical prices, EXPRESS Chemically Competent Cells offer superior value for everyday protein expression work.
New England Biolabs, Inc. Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) – 10 reactions
The Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours). The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid (5 minutes), room temperature circularization and template removal. Transformation into high-efficiency chemically-competent E. coli, not supplied, ensures robust results with plasmids up to at least 20 kb in length. Kit is also available with competent cells .
Zymo Research Corporation Mix & Go! Competent Cells - DH5 Alpha, 10 x 100 µl
Mix & Go E. coli cells are made chemically competent by a unique method that completely eliminates the need for heat shock and related procedures. For transformation, DNA can be added directly to Mix & Go cells and the mixture spread directly to a culture plate. Transformation efficiencies typically range from 108-109 transformants/µg of pUC19 DNA , which make the cells optimal for cloning, sub-cloning, library construction, etc. Premade Mix & Go cells are supplied as a pack of 10 convenient 100 µl/tube aliquots or in a 96-well format (12 x 8 - tube strips) of 50 µl/tube.
BIOSEARCH TECHNOLOGIES INC OverExpress ChemComboPack, 12 Rxns (SOLOs)
Express difficult or toxic proteins using standard E. coli T7 expression vectors using these unique strains. Some strains also available in electrocompetent format.Lucigens OverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3).
New England Biolabs, Inc. NEBuilder HiFi DNA Assembly Bundle for Large Fragments – 20 reactions
NEB recommends NEB 10-beta Competent E. coli , High Efficiency for assemblies larger than 15 kb. NEBuilder HiFi DNA Assembly Bundle for Large Fragments offers a discount for purchasing these competent cells with the NEBuilder HiFi DNA Assembly Master Mix. NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15-30 bp). It has utility for the synthetic biology community, as well as those interested in one-step cloning of multiple fragments due to its ease of use, flexibility and simple master-mix format.
New England Biolabs, Inc. Q5 Site-Directed Mutagenesis Kit – 10 reactions
The Q5 Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid (5 minutes), room temperature circularization and template removal. Transformation into high-efficiency chemically-competent E. coli, ensures robust results with plasmids up to at least 20 kb in length.