Biotinylated Horse Anti-Mouse IgG Antibody, Rat Adsorbed, is designed for use with rat tissues that may contain endogenous rat immunoglobulins. The antibodies are raised using proprietary immunization schedules that produce high affinity antibodies, then purified by affinity chromatography to remove cross-reactive antibodies that are likely to interfere with specific staining. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots. Biotinylated Horse Anti-Mouse IgG Antibody is supplied in solution and recognizes both heavy and light chains. With some exceptions, the recommended dilution for most applications is 1:100.