Standard PCR Reagents and Kits
Standard PCR Reagents and Kits
New England Biolabs, Inc. LongAmp Taq PCR Kit – 100 reactions (50 µl vol)
The LongAmp Taq PCR Kit features LongAmp Taq DNA Polymerase, which is a unique blend of Taq and Deep Vent DNA Polymerases. The 35 exonuclease activity of Deep Vent Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. LongAmp Taq DNA Polymerase offers two fold higher fidelity than Taq Polymerase alone. The LongAmp Taq PCR Kit is supplied with LongAmp Taq Reaction Buffer, which is optimized to give highest PCR yields up to 30 kb. The kit can generate a wide range of PCR products, up to 30 kb from plasmid or from human genomic DNA.
New England Biolabs, Inc. Deep Vent™ DNA Polymerase – 200 units
Deep Vent DNA Polymerase was the second high-fidelity thermophilic DNA polymerase available from New England Biolabs. The fidelity of Deep Vent DNA Polymerase is derived in part from an integral 3 5 proofreading exonuclease activity. Deep Vent is even more stable than Vent at temperatures of 95 to 100C.
New England Biolabs, Inc. Multiplex PCR 5X Master Mix – 100 reactions (50 µl vol)
Multiplex PCR can simultaneously detect two or more products in a single reaction. There is an increasing demand for multiplex PCR techniques in assays conducted in research laboratories and forensic/diagnostic genotyping assays. Multiplex PCR can also be used for semi-quantitative gene expression analysis using cDNA templates. The NEB Multiplex PCR 5X Master Mix is an easy-to-use solution featuring high quality recombinant Taq DNA Polymerase. The mix is optimized for high yield and robust performance. Its performance is illustrated in a 15-plex PCR using human genomic DNA and an 8-plex PCR using cDNA products as templates. The 5X formulation allows the user maximal input of primers, template DNAs and additional components.
New England Biolabs, Inc. Monarch PCR & DNA Cleanup Kit (5 µg) – 50 preps
The Monarch PCR and DNA Cleanup Kit rapidly and reliably purifies up to 5 ug of concentrated, high-quality DNA from PCR and other enzymatic reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 ul. The buffers provided have been optimized, and do not require monitoring of pH. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging. The protocol can also be modified to enable the purification of smaller DNA fragments, including oligonucleotides and ssDNA.