High Fidelity PCR Reagents and Kits
High Fidelity PCR Reagents and Kits
New England Biolabs, Inc. Q5 High-Fidelity PCR Kit – 50 reactions
Q5 High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance. This kit contains a sufficient supply of master mix and nuclease-free water to perform 50 polymerase chain reactions or 200 reactions. For added convenience, gel loading dye, and a Quick-Load DNA LAdder are also included in this kit.
New England Biolabs, Inc. Phusion High-Fidelity PCR Kit – 200 reactions
Phusion High-Fidelity DNA Polymerase offers both high-fidelity and robust performance, and thus can be used for all PCR applifications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5' to 3' polymerase activity, 3' to 5' exonuclease activity and will generate blunt-ended products. The Phusion High-Fidelity PCR Kit contains a sufficient supply of Phusion High-Fidelity DNA Polymerase, Phusion HF and GC Buffers, deoxynucleotides, magnesium chloride, DMSO, and DNA size standard to perform 50 reactions or 200 reactions.
New England Biolabs, Inc. Q5 High-Fidelity 2X Master Mix – 500 reactions (50 µl vol)
The Q5 High-Fidelity 2X Master Mix offers robust, high-fidelity performance in a convenient master mix format. The Q5 High- Fidelity 2X Master Mix features a high-fidelity, thermostable DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. The convenient master mix formulation is supplied at a 2X concentration. The mix contains dNTPs, Mg++ and a proprietary broad-use buffer requiring only the addition of primers and DNA template for robust amplification regardless of GC content. When used at the recommended 1X final concentration, the Q5 High-Fidelity Master Mix contains 2 mM Mg++. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.
New England Biolabs, Inc. Q5 Blood Direct 2X Master Mix - 100 rxns
Q5 Blood Direct 2X Master Mix can amplify a wide variety of targets direct from dried blood spots to up to 30% whole human blood skipping DNA purification. The master mix includes polymerase and dNTPs in an optimized buffer with increased resistance to inhibitors in blood anti-coagulants and chemicals on filter papers. It is capable of amplifying products up to 7.5 kb from human whole blood cells preserved with Na EDTA K2 EDTA Na citrate and Na heparin as well samples stored on common preservative filter papers. The master mix features Q5 Hot Start High-Fidelity DNA Polymerase a thermostable DNA polymerase with 3 prime to 5 prime exonuclease activity fused to a processivity-enhancing Sso7d domain for robust DNA amplification with low error rates. The addition of an aptamer-based inhibitor allows for room temperature reaction setup.
New England Biolabs, Inc. Q5 Blood Direct 2X Master Mix - 100 rxns
Q5 Blood Direct 2X Master Mix can amplify a wide variety of targets direct from dried blood spots to up to 30% whole human blood skipping DNA purification. The master mix includes polymerase and dNTPs in an optimized buffer with increased resistance to inhibitors in blood anti-coagulants and chemicals on filter papers. It is capable of amplifying products up to 7.5 kb from human whole blood cells preserved with Na EDTA K2 EDTA Na citrate and Na heparin as well samples stored on common preservative filter papers. The master mix features Q5 Hot Start High-Fidelity DNA Polymerase a thermostable DNA polymerase with 3 prime to 5 prime exonuclease activity fused to a processivity-enhancing Sso7d domain for robust DNA amplification with low error rates. The addition of an aptamer-based inhibitor allows for room temperature reaction setup.
New England Biolabs, Inc. Q5 Hot Start High-Fidelity 2X Master Mix – 500 rxn (1 x 12.5 ml)
The Q5 Hot Start High-Fidelity 2X Master Mix features a high-fidelity, thermostable, hot start DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. The addition of an aptamer-based inhibitor allows room temperature reaction setup. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 Hot Start High-Fidelity 2X Master Mix is ideal for cloning and can be used for long or difficult amplicons. The convenient master mix formulation is supplied at a 2X concentration. The mix contains dNTPs, Mg++ and a proprietary broad-use buffer requiring only the addition of primers and DNA template for robust amplification regardless of GC content. When used at the recommended 1X final concentration, the Q5 Hot Start High-Fidelity Master Mix contains 2 mM Mg++. Q5 Hot Start High-Fidelity 2X Master Mix is unlike typical, lower fidelity PCR enzymes.
New England Biolabs, Inc. Phusion High-Fidelity PCR Master Mix with HF Buffer – 500 reactions (50 µl vol)
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerase available. It possesses 5' to 3' polymerase activity, 3' to 5' exonuclease activity and will generate blunt-ended products. Phusion High-Fidelity PCR Master Mix with HF Buffer is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl2.
New England Biolabs, Inc. Phusion High-Fidelity PCR Master Mix with HF Buffer – 100 reactions (50 µl vol)
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerase available. It possesses 5' to 3' polymerase activity, 3' to 5' exonuclease activity and will generate blunt-ended products. Phusion High-Fidelity PCR Master Mix with HF Buffer is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl2.
New England Biolabs, Inc. Phusion High-Fidelity PCR Master Mix with GC Buffer – 500 reactions (50 µl vol)
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerase available. It possesses 5' to 3' polymerase activity, 3' to 5' exonuclease activity and will generate blunt-ended products. Phusion High-Fidelity PCR Master Mix with GC Buffer is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl2.
New England Biolabs, Inc. Q5 High-Fidelity 2X Master Mix – 100 reactions (50 µl vol)
The Q5 High-Fidelity 2X Master Mix offers robust, high-fidelity performance in a convenient master mix format. The Q5 High- Fidelity 2X Master Mix features a high-fidelity, thermostable DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. The convenient master mix formulation is supplied at a 2X concentration. The mix contains dNTPs, Mg++ and a proprietary broad-use buffer requiring only the addition of primers and DNA template for robust amplification regardless of GC content. When used at the recommended 1X final concentration, the Q5 High-Fidelity Master Mix contains 2 mM Mg++. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.
New England Biolabs, Inc. Q5 Hot Start High-Fidelity 2X Master Mix – 100 rxn (2 x 1.25 ml)
The Q5 Hot Start High-Fidelity 2X Master Mix features a high-fidelity, thermostable, hot start DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. The addition of an aptamer-based inhibitor allows room temperature reaction setup. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 Hot Start High-Fidelity 2X Master Mix is ideal for cloning and can be used for long or difficult amplicons. The convenient master mix formulation is supplied at a 2X concentration. The mix contains dNTPs, Mg++ and a proprietary broad-use buffer requiring only the addition of primers and DNA template for robust amplification regardless of GC content. When used at the recommended 1X final concentration, the Q5 Hot Start High-Fidelity Master Mix contains 2 mM Mg++. Q5 Hot Start High-Fidelity 2X Master Mix is unlike typical, lower fidelity PCR enzymes.
New England Biolabs, Inc. Phusion High-Fidelity PCR Kit – 50 reactions
Phusion High-Fidelity DNA Polymerase offers both high-fidelity and robust performance, and thus can be used for all PCR applifications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5' to 3' polymerase activity, 3' to 5' exonuclease activity and will generate blunt-ended products. The Phusion High-Fidelity PCR Kit contains a sufficient supply of Phusion High-Fidelity DNA Polymerase, Phusion HF and GC Buffers, deoxynucleotides, magnesium chloride, DMSO, and DNA size standard to perform 50 reactions or 200 reactions.
New England Biolabs, Inc. Phusion High-Fidelity DNA Polymerase – 100 units
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5' to 3' polymerase activity, 3' to 5' exonuclease activity and will generate blunt-ended products. Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. Each of these buffers contains MgCl2 (1.5 mM at the final [1X] reaction concentration).
New England Biolabs, Inc. NEBNext High-Fidelity 2X PCR Master Mix – 50 reactions
NEBNext High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent.
New England Biolabs, Inc. Phusion High-Fidelity DNA Polymerase – 500 units
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5' to 3' polymerase activity, 3' to 5' exonuclease activity and will generate blunt-ended products. Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. Each of these buffers contains MgCl2 (1.5 mM at the final [1X] reaction concentration).
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