Used in Wolbach's Giemsa Method, Wolbach, Todd, and Palfrey (1922) for the staining of : nuclei, collagen, riskettsia and bacteria. See references Wolbach, S.B., Todd, J.L., and Paltrey, F.W., The Etiology of Pathology Typhus, Harvard University Press, Cambridge, MA, p. 13-14, c. 1922 and Luna, L.G., (ed). Manual of Histologic Staining Methods od the AFIP, 3rd edition. McGraw-Hill, NY, p. 119, c. 1968.