Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

1 – 15 of 2,944 results

PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™

Commonly used buffer

Pricing & Availability
Specifications

Content And Storage	Room temperature
Form	Liquid

Phosphate Buffered Saline (PBS), 10X Powder, pH 7.4, Fisher BioReagents™

Phosphate Buffered Saline (PBS), 10X Powder provides a convenient way to make 1 L of 10X stock PBS solution.

Pricing & Availability

Tris Hydrochloride, 1M Solution (pH 8.0/Mol. Biol.), Fisher BioReagents™

CAS: 77-86-1,1185-53-1,7732-18-5 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris-HCl PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1,1185-53-1,7732-18-5
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
SMILES	C(C(CO)(CO)N)O
Synonym	Tris-HCl
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

TBS, Tris Buffered Saline, 1X Solution, pH 7.4, Molecular Biology, Fisher BioReagents

Pricing & Availability
Specifications

Boiling Point	100°C
Name Note	1X Solution, pH 7.4
CAS	77-86-1
Protease	Protease free
Color	Colorless
Physical Form	Liquid
Chemical Name or Material	Tris Buffered Saline
Grade	Molecular Biology
Synonym	TBS
DNase	DNase free
Recommended Storage	RT
ChemAlert Storage Symbol	Gray

TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents

Pricing & Availability
Specifications

Color	Colorless
Physical Form	Liquid
Chemical Name or Material	Tris-EDTA
Grade	Molecular Biology
DNase	DNase free
Filtered Through	Filtered through a 5-micron filter.
ChemAlert Storage Symbol	Gray
Name Note	1X Solution, pH 8.0
CAS	7732-18-5
Protease	Protease free
Health Hazard 3	Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0
Health Hazard 2	CAUTION!
pH	7.4 to 8.1
Synonym	TE
Recommended Storage	RT

PBS Tablets, Phosphate Buffered Saline, Fisher BioReagents™

Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!

Pricing & Availability

HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents™

Commonly used buffering agent

Pricing & Availability
Specifications

Color	Undesignated
Physical Form	Liquid
Chemical Name or Material	HEPES Buffer
Grade	Molecular Biology
Identification	Pass Test
DNase	DNase free
Merck Index	15, 4689
Concentration	0.95 to 1.05 M
ChemAlert Storage Symbol	Gray
Absorbance	≤0.01(0.1M solution) at 260nm,0.004 max. (0.1M solution) at 280nm
Name Note	1M Solution, pH 7.3
CAS	7732-18-5
Protease	Protease free
pH	7.2 to 7.5
Synonym	N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid
Purity Grade Notes	DNase-, RNase- and Protease-Free
Recommended Storage	RT
Molecular Formula	C₈H₁₈N₂O₄S
Formula Weight	238.197

Tris-Glycine-SDS, 10X Solution (Electrophoresis), Fisher BioReagents™

Pricing & Availability
Specifications

Color	Undesignated
Physical Form	Liquid
Chemical Name or Material	Tris-Glycine-SDS
Grade	Electrophoresis
Identification	Pass Test
DNase	DNase free
Filtered Through	Filtered through a 0.2-micron filter.
ChemAlert Storage Symbol	Gray
Name Note	10X Solution
Concentration or Composition (by Analyte or Components)	0.25M Tris Base, 1.92M Glycine, and 1.0% (w/v) SDS.
CAS	151-21-3
Protease	Protease free
Synonym	TG-SDS
Purity Grade Notes	DNase-, RNase- and Protease-Free
Recommended Storage	RT

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™

Commonly used buffering agent.

Pricing & Availability
Specifications

Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
Color	White
Chemical Name or Material	HEPES
Grade	Molecular Biology
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
Identification	Pass Test
DNase	DNase free
Merck Index	15, 4689
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
ChemAlert Storage Symbol	Gray
Assay Percent Range	≥99 %
PubChem CID	23831
Absorbance	0.01 max. (0.1M solution) at 280nm
Percent Purity	≥99%
CAS	7365-45-9
Protease	Protease free
Health Hazard 3	Emergency Overview Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. . NFPA Health:2 Flammability:1 Instability:1
MDL Number	MFCD00006158
Health Hazard 2	WARNING!
Solubility Information	Soluble in water
pH	5.0 to 6.5
Purity Grade Notes	DNase-, RNase- and Protease-Free
Recommended Storage	RT
Molecular Formula	C8H18N2O4S

Tris-Glycine, 10X Solution (Electrophoresis), Fisher BioReagents™

Pricing & Availability
Specifications

Color	Undesignated
Physical Form	Liquid
Chemical Name or Material	Tris-Glycine
Grade	Electrophoresis
DNase	DNase free
Filtered Through	Filtered through a 0.2-micron filter.
ChemAlert Storage Symbol	Gray
Name Note	10X Solution
Concentration or Composition (by Analyte or Components)	Components: Tris (2-3%) and Glycine (13-14%). 0.25M Tris Base and 1.92M Glycine.
CAS	56-40-6
Protease	Protease free
Synonym	TG
Purity Grade Notes	DNase-, RNase- and Protease-Free
Recommended Storage	RT

TE Buffer, Tris-EDTA, 100X Solution, pH 8.0, Molecular Biology, Fisher BioReagents™

Pricing & Availability
Specifications

Name Note	100X Solution
CAS	7732-18-5
Protease	Protease free
Health Hazard 3	Emergency Overview Irritating to eyes and skin. Wear personal protective equipment. Ensure adequate ventilation. Avoid contact with skin, eyes and clothing. Avoid ingestion and inhalation. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Get medical attention immediately if symptoms occur. Do not induce vomiting. NFPA Health:2 Flammability:0 Instability:0
Health Hazard 2	WARNING!
Color	Undesignated
Physical Form	Liquid
Chemical Name or Material	Tris-EDTA
Grade	Molecular Biology
DNase	DNase free
Recommended Storage	RT
Filtered Through	Filtered through a 0.2-micron filter.
ChemAlert Storage Symbol	Gray

TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™

CAS: 7732-18-5,77-86-1,60-00-4,10043-35-3 Synonym: TBE

Pricing & Availability
Specifications

CAS	7732-18-5,77-86-1,60-00-4,10043-35-3
Synonym	TBE

Tris Hydrochloride (Small White Flakes/Molecular Biology), Fisher BioReagents

CAS: 5704-04-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N Synonym: Tris(hydroxymethyl)aminomethane Hydrochloride PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl

Pricing & Availability
Specifications

PubChem CID	93573
CAS	5704-04-1
Molecular Weight (g/mol)	157.594
SMILES	C(C(CO)(CO)N)O.Cl
Synonym	Tris(hydroxymethyl)aminomethane Hydrochloride
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride
InChI Key	QKNYBSVHEMOAJP-UHFFFAOYSA-N
Molecular Formula	C4H12ClNO3

Tris Hydrochloride, 1M Solution (pH 7.5/Mol. Biol.), Fisher BioReagents™

CAS: 7732-18-5,77-86-1,1185-53-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris-HCl PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	7732-18-5,77-86-1,1185-53-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
SMILES	C(C(CO)(CO)N)O
Synonym	Tris-HCl
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

Tris (Tromethamine) (Granular/USP), Fisher Chemical™

CAS: 7646-85-7 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trishydroxymethylaminomethane,Tris Buffer,Tris,THAM,Trizma™,2-Amino-2-(hydroxymethyl)-1,3-propanediol,Tris base,Tris(hydroxymethyl)aminomethane,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	7646-85-7
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
Synonym	Trishydroxymethylaminomethane,Tris Buffer,Tris,THAM,Trizma™,2-Amino-2-(hydroxymethyl)-1,3-propanediol,Tris base,Tris(hydroxymethyl)aminomethane,Trometamol
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

Life Science Buffers

Life Science Buffers

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