Staining Reagents
Staining Reagents
Dojindo Molecular Technologies Inc FerroOrange (1 TUBE, 24UGx1), novel fluorescent probe for the detection of high sensitivity for intracellular Fe2+ detection, used in ferroptosis research. live-cell imaging, applicable for plate reader
Iron is the most abundant transition metal element within organisms, and it participates in various physiological activities. Free iron in living cells is particularly important because of its high reactivity, suggesting a relationship to cellular damage or death. Free iron exists in its stable redox states, ferrous ion (Fe2+) and ferric ion (Fe3+). In living cells, research involving this behavior is necessary to understand the intracellular reductive environment, metal transporters and water solubility of Fe2+. FerroOrange is a novel fluorescent probe that enables live-cell fluorescent imaging with fluorescence microscopy and quantitative assay by plate reader. Dojindo has various reagents for ferroptosis research. ( https://www.dojindo.com/ferroptosis-detection-selection-guide/)
Dojindo Molecular Technologies Inc FerroOrange (3 TUBE, 24UGx3), novel fluorescent probe for the detection of high sensitivity for intracellular Fe2+ detection, used in ferroptosis research. live-cell imaging, applicable for plate reader
Iron is the most abundant transition metal element within organisms, and it participates in various physiological activities. Free iron in living cells is particularly important because of its high reactivity, suggesting a relationship to cellular damage or death. Free iron exists in its stable redox states, ferrous ion (Fe2+) and ferric ion (Fe3+). In living cells, research involving this behavior is necessary to understand the intracellular reductive environment, metal transporters and water solubility of Fe2+. FerroOrange is a novel fluorescent probe that enables live-cell fluorescent imaging with fluorescence microscopy and quantitative assay by plate reader. Dojindo has various reagents for ferroptosis research. ( https://www.dojindo.com/ferroptosis-detection-selection-guide/)
Revvity Health Sciences Inc Cat B 680 FAST Fluorescent Imaging Agent
Cat B 680 FAST™ is a member of a family of activatable fluorescent imaging agents comprising a novel architecture, termed F.A.S.T. (Fluorescent Activatable Sensor Technology) that confers an improved pharmacokinetic profile with a broader range of early imaging time points. This architecture also offers higher target specific signal with reduced background.¿ Cat B 680 FAST is a Cathepsin B activatable fluorescent agent that is optically silent upon injection and produces fluorescent signal after cleavage by Cathepsin B produced by inflammatory cells and tumor cells. Cat B 680 FAST may be used to monitor inflammation, tumor activity, progression of disease and the efficacy of therapeutic treatment in applications such as oncology, inflammation, cardiovascular disease and some neurological diseases.
Biotium CellBrite Cytoplasmic Membrane Dyes
CellBrite Cytoplasmic Membrane Dyes are a set of fluorescent membrane dyes used to stain the plasma membrane of live or fixed cells for microscopy applications. They produce bright staining of the plasma membrane with low background fluorescence. The dyes are available in green, orange, deep red, and near-infrared fluorescent colors. They are compatible with common filter sets and have high photostability. The dyes can be used for various applications including cell tracking, cell proliferation studies, and co-localization experiments. They are supplied as DMSO solutions and are non-toxic to cells at working concentrations.
STEMCELL Technologies Propidium Iodide, Size: 10 mg
Propidium Iodide (PI) is a red-fluorescent cell viability dye which is excluded from live cells with intact membranes, but penetrates dead or damaged cells and binds to DNA and RNA by intercalating between the bases. It is widely used as a counterstain to differentiate and exclude non-viable cells in flow cytometric analyses, and can be excited using blue (488 nm), green (532 nm), or yellow-green (561 nm) laser lines, with detection in the FL2 or FL3 channels. PI is used in DNA fluorescence imaging applications to discriminate early and late stages of apoptosis, to study cell‐mediated cytotoxicity, and for chromosome analysis. It is also commonly used in quantitative DNA assays.
Biotium RedDot™2, 200X in DMSO
RedDot™2 is a far-red cell membrane-impermeant nuclear dye similar to Draq7™. Ideal for staining the nuclei of dead or fixed and permeabilized cells with minimal cytoplasmic RNA staining. The dye is highly thermostable and photostable, providing convenient handling and ideal for demanding applications such as confocal microscopy. The dye has an excitation/emission of 665/695 nm when bound to DNA. Properties: Liquid; Store at 4°C, protect from light; Ex/Em= 665/695 nm (with DNA).
Biotium REDDOT2 FAR RED NUCLEAR STAIN
RedDot™2 is a far-red cell membrane-impermeable nuclear dye similar to Draq7™ with excitation/emission at 665/695 nm. The dye is ideal for specifically staining the nuclei of dead cells with minimal cytoplasmic RNA staining. RedDot™2 provides excellent nuclear counterstaining in fixed and permeabilized cells and tissue sections without the need for RNase treatment to remove cytoplasmic RNA. The dye is highly thermostable and photostable, providing convenient handling and ideal for demanding applications such as confocal microscopy. RedDot™2 also has been used for cell number normalization for near-infrared In Cell Western™. The excitation maximum of RedDot™2 is 665 nm, but it can be efficiently excited by wavelengths from 488 to 647 nm, and therefore can be used with the 488 nm flow cytometry laser line. Supplied as a 200X stock solution in DMSO, 250 uL. In Cell Western is a trademark of LI-COR® Biosciences.
Biotium REDDOT2 FAR-RED NUCLEAR STAIN
RedDot™2 is a far-red cell membrane-impermeable nuclear dye similar to Draq7™ with excitation/emission at 665/695 nm. The dye is ideal for specifically staining the nuclei of dead cells with minimal cytoplasmic RNA staining. RedDot™2 provides excellent nuclear counterstaining in fixed and permeabilized cells and tissue sections without the need for RNase treatment to remove cytoplasmic RNA. The dye is highly thermostable and photostable, providing convenient handling and ideal for demanding applications such as confocal microscopy. RedDot™2 also has been used for cell number normalization for near-infrared In Cell Western™. The excitation maximum of RedDot™2 is 665 nm, but it can be efficiently excited by wavelengths from 488 to 647 nm, and therefore can be used with the 488 nm flow cytometry laser line. Supplied as a 200X stock solution in DMSO, 25 uL trial size. In Cell Western is a trademark of LI-COR® Biosciences.
Biotium CELLBRITE BLUE CYTO MEM LAB KI
Biotium’s CellBrite™ Cytoplasmic Membrane Labeling Dyes are lipophilic carbocyanine dyes that label cytoplasmic membrane and intracellular membrane structures efficiently and permanently. They have been used as tracers in cell–cell fusion, cellular adhesion, and migration applications due to their properties of low cytotoxicity and high resistance to intercellular transfer. CellBrite™ dyes are dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture. The CellBrite™ Blue Cytoplasmic Membrane Labeing Kit features DiB, the first blue carbocyanine dye, which has excitaiton/emission at 366/441 nm in membranes. Biotium offers a selection of CellBrite™ dyes to allow cell populations to be marked in distinctive fluorescent colors for identification after mixing. Double labeling can identify cells that have fused or formed stable clusters. Kit contents: 250 uL DiB cell labeling solution and 250 uL DiB loading buffer.
Biotium CellBrite Cytoplasmic Membrane Dyes
Biotium’s CellBrite™ Cytoplasmic Membrane Labeling Dyes are lipophilic carbocyanine dyes that label cytoplasmic membrane and intracellular membrane structures efficiently and permanently. They have been used as tracers in cell–cell fusion, cellular adhesion, and migration applications due to their properties of low cytotoxicity and high resistance to intercellular transfer. CellBrite™ dyes are dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture. CellBrite™ Orange Cytoplasmic Membrane Labeing Dye features orange-red fluorescent DiI, which has excitaiton/emission at 549/565 nm in membranes. Biotium offers a selection of CellBrite™ dyes to allow cell populations to be marked in distinctive fluorescent colors for identification after mixing. Double labeling can identify cells that have fused or formed stable clusters. 1 mL labeling solution.
Biotium CELLBRITE GREEN CYTOPLASMIC
Biotium’s CellBrite™ Cytoplasmic Membrane Labeling Dyes are lipophilic carbocyanine dyes that label cytoplasmic membrane and intracellular membrane structures efficiently and permanently. They have been used as tracers in cell–cell fusion, cellular adhesion, and migration applications due to their properties of low cytotoxicity and high resistance to intercellular transfer. CellBrite™ dyes are dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture. CellBrite™ Green Cytoplasmic Membrane Labeing Dye features green fluorescent Neuro-DiO, which has excitaiton/emission at 484/501 nm in membranes. Biotium offers a selection of CellBrite™ dyes to allow cell populations to be marked in distinctive fluorescent colors for identification after mixing. Double labeling can identify cells that have fused or formed stable clusters. 1 mL labeling solution.
Biotium RedDot™1, 200X in water
RedDot™1 is a far-red cell membrane-permeable nuclear dye similar to Draq5™. The dye is ideal for specifically staining the nuclei of live cells. RedDot™1 is highly thermostable and photostable, providing convenient handling for demanding applications such as confocal microscopy. The stain may also be used for DNA content analysis by flow cytometry. The dye has an excitation/emission of 662/694 nm when bound to DNA. Properties: Liquid; Store at 4°C or -20°C, protect from light; Ex/Em= 662/694 nm (with DNA).
Biotium RedDot™1, 200X in water
RedDot™1 is a far-red cell membrane-permeable nuclear dye similar to Draq5™. The dye is ideal for specifically staining the nuclei of live cells. RedDot™1 is highly thermostable and photostable, providing convenient handling for demanding applications such as confocal microscopy. The stain may also be used for DNA content analysis by flow cytometry. The dye has an excitation/emission of 662/694 nm when bound to DNA. Properties: Liquid; Store at 4°C or -20°C, protect from light; Ex/Em= 662/694 nm (with DNA).
Biotium RedDot™1, 200X in water
RedDot™1 is a far-red cell membrane-permeable nuclear dye similar to Draq5™. The dye is ideal for specifically staining the nuclei of live cells. RedDot™1 is highly thermostable and photostable, providing convenient handling for demanding applications such as confocal microscopy. The stain may also be used for DNA content analysis by flow cytometry. The dye has an excitation/emission of 662/694 nm when bound to DNA. Properties: Liquid; Store at 4°C or -20°C, protect from light; Ex/Em= 662/694 nm (with DNA).
Biotium JC-1 (chloride salt)
JC-1 (5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine chloride) is a live cell membrane potential-dependent mitochondrial dye. A ratiometric dye, JC-1 monomers (green fluorescence, Em = 527 nm) are in equilibrium with so-called J-aggregates (red fluorescence, Em = 590nm) that are favored at higher dye concentrations or higher mitochondrial membrane potential. The green/red fluorescence ratio can therefore be used as a measure of mitochondrial membrane potential. JC-1 is particularly useful for apoptosis studies. In apoptotic cells, the dye stays in the cytoplasm and fluoresces green. It is also used in high-throughput drug screening applications. JC-1, iodide is the original salt form of the dye. JC-1, chloride is preferable for applications where the iodide ion is not desired, while also being potentially more water soluble.Properties: e (MeOH) = 190,000; Red solid soluble in DMSO; Store at 4°C; Protect from light, especially in solution; C25H27Cl5N4; MW: 561.5
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