Applied Biosystems™ TaqMan™ Genotyping Master Mix

Optimized for end-point fluorescence detection in SNP genotyping applications.

Supplier:  Applied Biosystems™ 4381657

Catalog No. 43-816-57

Description

• Distinct clusters and high call rates for unambiguous allelic discrimination
• Validated with TaqMan™ SNP Genotyping Assays
• Excellent pre- and post-PCR stability for high throughput setup and analysis
• Reaction size is based on a 50μL reaction volume

May be used for a variety of genotyping applications, including:

• Candidate gene studies
• Drug-target validation
• Disease association studies
• Population genetics
• Linkage mapping
• Agricultural applications

Exceptional Performance from an Optimized Mix (2X)
Accurate genotype assignments result from preferential binding of the allele-specific probe to the matching target. TaqMan™ Genotyping Master Mix enables specific binding of the probe to achieve exceptional cluster resolution. Key features include:

• AmpliTaq Gold™ DNA Polymerase, UP (Ultra Pure) - Automatic hot start enzyme designed to be active during thermal cycling and inactive at room temperature for easy reaction setup
• Optimized mix components provide excellent specificity for discrimination between alleles
• Passive internal reference based on proprietary ROX™ dye for increased precision on Applied Biosystems™ real-time PCR instruments
• Single thermal cycling condition for consistent results with TaqMan™ Assays

Validated with TaqMan™ Genotyping Assays and Instruments:

• TaqMan™ Genotyping Master Mix has been tested across all types of TaqMan™ SNP Genotyping Assays: TaqMan™ Drug Metabolism Genotyping Assays, TaqMan™ SNP Genotyping Assays, and Custom TaqMan™ SNP Genotyping Assays
• In addition, the master mix is validated with the Applied Biosystems™ thermal cyclers and real-time PCR systems

High-Throughput Setup and Analysis:

• The combination of several low-cost thermal cyclers for PCR and a single real-time PCR instrument for allelic discrimination make high throughput SNP genotyping manageable
• TaqMan™ Genotyping Master Mix further improves high-throughput setup and analysis with excellent room temperature stability both before and after PCR
• This provides the flexibility required to run long experiments, unattended overnight or over a weekend

Reliable Discrimination with Challenging Targets

• Amplicon design constraints make SNP detection challenging, particularly when targets contain an abundance of GC- or AT-rich regions
• The optimized components of TaqMan™ Genotyping Master Mix result in tight, well-separated clusters and more accurate allele-calls compared to other mixes, even for challenging targets

Setting the Industry Standard for Quality:

• Extensive analytical and functional tests are performed for each manufactured lot of TaqMan™ Genotyping Master Mix, including one TaqMan™ SNP Genotyping Assay and one TaqMan™ Drug Metabolism Genotyping Assay
• Results are compiled in an informative Certificate of Analysis, which is accessible through the Applied Biosystems™ Web site

Order Info

Guaranteed minimum shelf life is 60 days. Shipping Conditions: Wet Ice

Specifications

• Content And Storage: Supplied at 2X concentration. The mix is optimized for SNP genotyping using TaqMan™ probes and contains AmpliTaq Gold™ DNA Polymerase UP (Ultra Pure), dNTPs without dUTP, Passive Reference 1 and optimized mix components. This pack contains two 50 mL bottles, sufficient for 4,000 reactions at the 50 μL total volume.
  
  Storage: 2-8°C
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
• GC-Rich PCR Performance: High
• Polymerase: AmpliTaq Gold DNA Polymerase
• Technique: SNP Genotyping (Real-Time PCR-Based), CNV (Copy Number Variation)
• Genotyping Target: Copy Number Variants, SNPs (Unknown or Numerous), Drug Metabolizing Enzymes, SNPs (Known)
• Product Line: TaqMan™
• Purity or Quality Grade: UP (Ultra Pure)
• For Use With (Application): Genotyping
• Fidelity (vs. Taq): 2 X
• Volume: 100 mL

• Detection Method: Primer-probe
• PCR Method: qPCR
• Reaction Speed: Standard
• For Use With (Equipment): Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7
• Passive Reference Dye: ROX (Pre-mixed)
• Product Type: Genotyping Master Mix
• Quantity: 2 x 50 mL
• Concentration: 2X
• Sample Type: DNA (Genomic)

For Research Use Only. Not for use in diagnostic procedures.