Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis

Supplier:  Thermo Scientific™ 34577

Catalog No. PI34577

Description

Thermo Scientific™ SuperSignal™ West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions making it an ideal choice for most western blotting applications.

Features of SuperSignal West Pico PLUS Chemiluminescent Substrate:

• Picogram to femtogram sensitivity—detect low-picogram to high-femtogram amounts of target protein on nitrocellulose or PVDF membrane
• High signal stability—incubated blots provide stable signal duration over the critical 4 hour time period with up to 24 hours of light output under optimal conditions
• Stable reagent—8-hour working solution stability; 1-year kit stability at room temperature
• Economical—optimized for dilute antibody concentrations:
  • 0.2 to 1.0μg/mL primary antibody (1:1000 to 1:5000 dilution from 1mg/mL stock)
  • 10 to 50ng/mL secondary antibody (1:20,000 to 1:100,000 dilution from 1mg/mL stock)
• Exceptional robustness—provides high performance outside of the recommended antibody dilution ranges, including the most common 1:5K to 1:10K secondary antibody dilutions of a 1mg/mL stock solution

For Research Use Only. Not for use in diagnostic procedures.

Specifications

• Product Type: Substrate
• Form: Liquid
• Membrane Compatibility: Nitrocellulose, PVDF
• Quantity: 200 mL
• Substrate: Horseradish Peroxidase
• Sensitivity: Picogram to high femtogram-level
• Sufficient For: 25 Mini-Gel Blots, 2000 cm² of Membrane
• Content And Storage: Sufficient for 25 mini-gel blots; 2000 cm² of membrane
  • SuperSignal West Pico PLUS Luminol/Enhancer, 100 mL
  • SuperSignal West Pico PLUS Stable Peroxide, 100 mL
  
  Store at room temperature.

• Detection Method: Chemiluminescence
• For Use With (Application): Western Blot
• Product Line: SuperSignal™
• Conjugate: HRP
• Recommended Antibody Concentrations: 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)
• Signal Duration: 6 to 24 hr.
• Kit Contents: SuperSignal West Pico PLUS Luminol/Enhancer, SuperSignal West Pico PLUS Stable Peroxide
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.