Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate

SuperSignal™ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.

Supplier:  Thermo Scientific™ A38554

Catalog No. PIA38554

Description

• Sensitivity: low-femtogram to high-attogram
• Stability: eight hours for working solution, one year for kit at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to four hours
• Recommended primary antibody concentration: 1:1000 to 1:5000 dilution (0.2 to 1.0 μg/mL)
• Recommended secondary antibody concentration: 1:100000 to 1:250000 dilution (4 to 10 ng/mL)

Specifications

• Product Type: Substrate
• Form: Liquid
• Membrane Compatibility: Nitrocellulose, PVDF
• Quantity: 20 mL
• Sensitivity: Low-femtogram to high-attogram
• Content And Storage: • Substrate Solution, 10 mL
  • Stable Peroxide Solution, 10 mL
  
  Sufficient for two mini-gel blots; 200 cm² of membrane.
  
  Store reagents at 2–8°C.
  
  

• Detection Method: Chemiluminescence
• For Use With (Application): Western Blot
• Product Line: SuperSignal™
• Substrate: Horseradish Peroxidase
• Sufficient For: 2 Mini-Gel Blots, 200 sq. cm of Membrane
• Shipping Condition: Wet Ice

For Research Use Only. Not for use in diagnostic procedures.