Invitrogen™ SuperScript™ IV RT-LAMP Master Mix

Invitrogen SuperScript IV RT-LAMP Master Mix is a solution designed for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions.

Supplier:  Invitrogen™ A51803

Catalog No. A51803

Description

• A solution designed for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions
• It allows for fast viral pathogen RNA amplification, providing high detection sensitivity, specificity, and robust performance
• The optimized master mix utilizes best-in-class enzymes that enable reliable target detection in 5 to 15 minutes for a variety of viral RNA targets and concentrations
• It includes an evolved Bst DNA polymerase mutant with high inhibitor tolerance and rapid target amplification at a temperature optimum of 65°C
• SuperScript IV Reverse Transcriptase features increased thermostability, speed, and inhibitor tolerance
• To prevent RNA degradation by carry-over RNases the master mix includes the potent, noncompetitive RNaseOUT Recombinant Ribonuclease Inhibitor
• The proprietary reaction buffer composition is optimized to increase specificity by delaying no-template control (NTC) amplification time
• SuperScript IV RT-LAMP Master Mix performance has been validated for multiple viral RNAs including measles, influenza, and SARS-CoV-2 RNA
• The master mix can be used to detect DNA with a reaction speed similar to or faster than that for RNA targets
• Available in a convenient single-tube format with SYTO 9 dye for real-time detection
• The master mix format offers flexibility to select and test different primers for desired RNA targets, and to use with end-point or real-time detection methods

Includes

• SuperScript IV RT-LAMP Master Mix (2X), 1.25 mL
• SYTO Green Fluorescent Nucleic Acid Stain, 30 μL

Specifications

• Product Type: Master Mix
• Content And Storage: • 10 x 1.25 mL SuperScript IV RT-LAMP Master Mix (2X)
  • 1 x 30 µL SYTO Green Fluorescent Nucleic Acid Stain
  
  Store at –20°C. Protect from light.
  
  
• Optimal Reaction Temperature: 65°C
• Reverse Transcriptase: SuperScript™ IV
• Includes Label or Dye: SYTO 9 Green Fluorescent Nucleic Acid Stain

• Polymerase: Bst DNA Polymerase
• No. of Reactions: 1,000 Reactions
• Reaction Speed: 5 to 15 minutes
• Detection Method: Real-time and End-point Detection
• Target Detection Validated: Viral, bacterial, fungal pathogens: SARS-CoV-2, Influenza, M. pneumoniae, human adenovirus, etc.