Thermo Scientific™ Pierce™ Recombinant Protein L, Peroxidase Conjugated

Detect mouse and human antibodies containing kappa light chains in immunoassays using this purified, biotinylated or peroxidase-conjugated, recombinant Protein L.

Supplier:  Thermo Scientific™ 32420

Catalog No. PI32420

Description

Purified and conjugated forms of Thermo Scientific™ Pierce Recombinant Protein L enable probing and detection of mouse and human antibodies containing kappa light chains in Western blotting, ELISA, IHC and other immunoassay protocols.

Protein L is an immunoglobulin-binding protein that was originally derived from the bacteria Peptostreptococcus magnus, but is now produced recombinantly. Protein L has the unique ability to bind through kappa light chain interactions without interfering with an antibody's antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L will bind to all classes of Ig (IgG, IgM, IgA, IgE and IgD). Protein L will also bind single chain variable fragments (scFv) and Fab fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse.

Highlights:

• Contains four immunoglobulin-binding domains per protein
• Binds members of all classes of Ig (IgG, IgM, IgA, IgE and IgD) that contain kappa light chains
• Binds single chain variable fragments (scFv) and Fab fragments that contain kappa light chains

Specifications

• Product Type: Protein L, Peroxidase Conjugate
• Quantity: 0.5 mg
• Form: Powder
• Target: Antibodies

• Conjugate: HRP
• Product Line: Pierce™
• Protein Subtype: Protein L
• Content And Storage: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20°C.