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Thermo Scientific™ PVDF Transfer Membranes, 0.45 μm

Pierce PVDF Transfer Membranes are made of high-quality polyvinylidene difluoride and provide high binding capacity for proteins and nucleic acids for western, Southern, and northern blotting methods.

Supplier:  Thermo Scientific™ 88518

Catalog No. PI88518


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Description

Description

Pierce PVDF Transfer Membranes are made of high-quality polyvinylidene difluoride and provide high binding capacity for proteins and nucleic acids for western, Southern, and northern blotting methods. The 0.45-μm pore size make these membranes well-suited for a wide range of western blotting conditions and ideal for transfer of proteins >10 kDa. For best results, preactivate the membrane with 100% alcohol.

Features include:
• Re-probe characteristics—high mechanical strength makes PVDF an excellent membrane for stripping and re-probing
• Durability—compatible with most organic solvents, acids, and mild bases
• Compatible with commonly used transfer conditions and detection methods such as staining, chemiluminescence, and radiolabeling
• High sensitivity—exceptional binding capacity makes PVDF the ultimate choice for low abundance proteins

Specifications

Specifications

3.75 m
Pierce PVDF Transfer Membrane, 0.45 μm, 3.75 m x 26.5 cm
1 Roll
PVDF
Store membranes flat at ambient temperature and away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure.
3.75 m x 26.5 cm
26.5 cm

294 μg/cm2
BSA: 215 μg/cm2
Insulin: 160 μg/cm2

Roll
0.45 μm
Room Temperature
≥100 Mini-gel Blots (When cut to 8.8 x 10 cm); ≥80 Midi-gel Blots (When cut to 8.5 x 13.25 cm)
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