Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate

Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

Catalog Number	Mfr. No.	Quantity	Sufficient For
FER32132X3	Thermo Scientific™ 32132X3	300 mL Kit	3000 sq. cm of membrane
FER32132	Thermo Scientific™ 32132	100 mL Kit	10 mini-gel blots; 1000cm2 of membrane

Description

Thermo Scientific™ Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated probes by Western blotting using X-ray film, CCD or laser-based imagers.

Pierce ECL Plus Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for Western blotting.

Highlights:

• Easy to use — can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization

• Higher sensitivity — detect targets down to the low-picogram level

• Longer signal duration — sustained light output for as long as five hours

• More imaging options — X-ray, CCD or laser-based imagers

• More affordable — high quality and performance at a lower price than other suppliers« similar substrates

Specifications

• Product Type: Substrate
• Form: Liquid
• Product Line: Pierce™

• Detection Method: Chemiluminescence, Fluorescence
• Membrane Compatibility: Nitrocellulose, PVDF
• Sensitivity: Low picogram to mid-femtogram

For Research Use Only. Not for use in diagnostic procedures.