Invitrogen™ NFkB p65 (Phospho) [pS536] Human InstantOne™ ELISA Kit

InstantOne ELISA

Supplier:  Invitrogen™ 858608211

Catalog No. 50-246-258

Includes:

• User Manual
• InstantOne™ Assay Plate - 96 well (sealed)
• Cell Lysis Buffer (5X)
• Enhancer Solution
• Wash Buffer (10X)
• phospho-NFkB p65 (Ser536) Capture Antibody Reagent
• phospho-NFkB p65 (Ser536) Detection Antibody Reagent
• Positive Control Cell Lysate (lyophilized)
• Detection Reagent (TMB Substrate)
• Stop Solution
• Plate Seal

Description

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human NFkB p65 in cell lysates. Detection of mouse and rat NFkB is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

NF-kappaB (Nuclear Factor kappa B) is a nuclear transcription factor found in all cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, and bacterial or viral antigens. NF-kappaB plays a key role in regulating the immune response to infection. Consistent with this role, incorrect regulation of NF-kappaB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection and improper immune development. There are five members in the NF-kappaB family: NF-kappaB1, NF-kappaB2, RelA (also named p65), RelB, and c-Rel. The most common form of NF-kappaB is the p50/RelA heterodimer, although other forms of NF-kappaB dimers, such as p50/p50, p52/p52, p52/RelA, p50/c-Rel, c-Rel/c-Rel, p52/RelB, and p50/RelB, have also been identified in some types of cells. The primary role of NF-kappaB is to maintain normal cellular functions that range from cell-to-cell communication to cell motility, cell cycle progression, and cell lineage development. The activity of NF-kappaB is tightly regulated by interaction with inhibitory IkappaB proteins.

Specifications

• Accession Number: Q04206
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: p65,NFKB3
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 1 hr. 10 min.

• Conjugate: Biotin
• Sample Type: Cell Lysates
• For Use With (Application): ELISA
• Gene ID (Entrez): 5970
• Kit Contents: Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
• Quantity: 96 Tests
• Sample Volume: Cell Lysate,20 μL
• Synonym: RELA, NFKB3, NFkappaB, p65
• Test Time: 1 hr. 20 min.

For Research Use Only