Invitrogen™ p70 S6 Kinase (Total) Multispecies InstantOne™ ELISA Kit

InstantOne ELISA

Supplier:  Invitrogen™ 858605111

Catalog No. 50-246-248

Includes: User Manual
InstantOne™ Assay Plate - 96 well (sealed)
Cell Lysis Buffer (5X)
Enhancer Solution
Wash Buffer (10X)
p70 S6K Capture Antibody Reagent
p70 S6K Detection Antibody Reagent
Positive Control Cell Lysate (lyophilized)
Detection Reagent (TMB Substrate)
Stop Solution
Plate Seal

Description

The InstantOne™ ELISA is specifically engineered for accurate measurement of total human and mouse p70 S6K in cell lysates. Detection of rat p70 S6K is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

The p70 S6 kinase (p70 Ribosomal Protein S6 Kinase, p70S6K) is a 70 kDa member of the ribosomal S6 kinase (RSK) family of serine/threonine kinases. p70 S6 kinase is predominantly localized in the cytoplasm, and is essential in growth factors regulated cell proliferation, pathways involving cell motility, such as metastases, the immune response, and tissue repair. p70 S6 kinase acts downstream of phosphoinositide (PI) 3-kinase, and its main physiological target is the S6 ribosomal protein, which is involved in upregulation of protein synthesis. The kinase activity of p70 S6 kinase leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding p70 S6 kinase and overexpression are seen in some breast cancer cell lines.

Specifications

• Accession Number: P23443,Q8BSK8
• Product Type: ELISA Kit
• Target Species: Human, Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: PS6K,S6K,S6K-beta-1,S6K1,STK14A,p70 S6KA,p70(S6K)-alpha,p70-S6K,p70-alpha
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human, Mouse
• Total Assay Time: 1 hr. 10 min.

• Conjugate: Biotin
• Sample Type: Cell Lysates
• For Use With (Application): ELISA
• Gene ID (Entrez): 6198, 72508
• Kit Contents: Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
• Quantity: 96 Tests
• Sample Volume: Cell Lysate,20 μL
• Synonym: Ribosomal protein S6 kinase beta-1, RPS6KB1, STK14A
• Test Time: 1 hr. 20 min.

For Research Use Only