Invitrogen™ VEGF-A Mouse ELISA Kit

For the quantitative detection of mouse VEGF-A.

Supplier:  Invitrogen™ BMS6192

Catalog No. 50-183-35

Includes: Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to mouse VEGF-A
Biotin-Conjugate anti-mouse VEGF-A polyclonal antibody
Streptavidin-HRP
Mouse VEGF-A Standard lyophilized, 4ng/mL upon reconstitution
Sample Diluent
Assay Diluent
Conjugate Diluent Concentrate 20x
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

VEGF (vascular endothelial growth factor) which is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. VEGF has a variety of effects on vascular endothelium, including the ability to promote endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability. The VEGF family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis, and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. Studies have shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis. Elevated levels of VEGF is linked to POEMS syndrome (Polyneuropathy, Organomegaly, Endocrinopathy, Monoclonal gammopathy, Skin changes) also known as Crow-Fukase syndrome which affects multiple organs in the body.

Specifications

• Accession Number: Q00731
• For Use With (Equipment): Colorimetric Microplate Reader
• Label or Dye: HRP
• For Use With (Application): ELISA
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Test Time: 1 hr. 20 min.
• Target: Mouse
• Interassay CV: 6.4%
• Quantity: 96 Tests
• Sample Type: Plasma, Serum, Supernatant
• Gene Symbol: Vegf,Vpf

• Product Type: ELISA Kit
• Conjugate: Biotin
• Assay Range: 62.5-4,000 pg/mL
• Gene ID (Entrez): 22339
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Total Assay Time: 4 hr. 30 min.
• Synonym: Vascular endothelial growth factor A, VPF
• Intraassay CV: 3.8%
• Target Species: Mouse
• Assay Sensitivity: 20.0 pg/mL

For Research Use Only