Invitrogen™ IL-2 Mouse ELISA Kit

For the quantitative detection of mouse IL-2.

Supplier:  Invitrogen™ BMS601

Catalog No. 50-182-80

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-2
Biotin-Conjugate anti-mouse IL-2 monoclonal antibody
Streptavidin-HRP
Mouse IL-2 Standard lyophilized, 2ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

The Mouse Interleukin-2 (Ms IL-2) ELISA quantitates Ms IL-2 in mouse serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-2. Principle of the method The Mouse IL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

Specifications

• Accession Number: P04351
• Assay Sensitivity: 5.3 pg/mL
• Product Type: ELISA Kit
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IL-2
• Intraassay CV: 5%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 3 hr. 10 min.

• Assay Range: 15.6-1,000 pg/mL
• Conjugate: Biotin
• Sample Type: Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 16183
• Interassay CV: 10%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Sample Volume: Serum, 50 μL; Supernatant, 50 μL
• Synonym: Aldesleukin, T cell growth factor, TCGF
• Test Time: 1 hr. 20 min.

For Research Use Only