Invitrogen™ IL-12 p70 Mouse ELISA Kit

For the quantitative detection of mouse IL-12 p70.

Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-12 p70
Biotin-Conjugate anti-mouse IL-12 p70 monoclonal antibody
Streptavidin-HRP
Mouse IL-12 p70 Standard lyophilized, 2ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Catalog Number	Mfr. No.	Quantity
50-182-70	Invitrogen™ BMS6004	96 Tests
50-182-71	Invitrogen™ BMS6004TEN	10 x 96 Tests

Description

The Mouse Interleukin-12 p70 (Ms IL-12 p70) ELISA quantitates Ms IL-12 p70 in mouse serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-12 p70. Principle of the method The Mouse IL-12 p70 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.

Specifications

• Accession Number: P43431,P43432
• Assay Sensitivity: 10.0 pg/mL
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IL-12p35,Il-12a,Ll12a,p35,Il-12b,Il-12p40,Il12p40,p40
• Intraassay CV: 8.3%
• Label or Dye: HRP
• Sample Volume: Serum, 50 μL; Supernatant, 50 μL
• Synonym: Interleukin-12 subunit p70, CLMF, NKSF
• Test Time: 1 hr. 20 min.

• Assay Range: 15.6-1,000 pg/mL
• Sample Type: Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 16159, 16160
• Interassay CV: 11%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 3 hr. 10 min.

For Research Use Only