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MilliporeSigma™ Magna Nuclear RIP™ (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation Kit
Specially designed to allow the discovery and analysis of a variety of chromatin associated RNAs such as long non-coding RNAs, enhancer RNAs and miRNAs
Supplier: MilliporeSigma™ 1710520
Includes:
- 10X Glycine
- 10X PBS
- Nuclei Isolation Buffer
- RIP Cross-Linked Lysis Buffer
- Protein A/G Magnetic Beads
- Nuclear RIP Dilution Buffer
- Low Salt Wash Buffer
- High Salt Wash Buffer
- LiCl Wash Buffer
- TE Buffer
- RIP Elution Buffer
- 10% SDS
- 0.5 M EDTA
- DNase I (RNase Free)
- DNase I Supplement
- DNase I Reaction Buffer
- Protease Inhibitor Cocktail III, Animal Free
- RNAse Inhibitor
- Proteinase K
Requires :
- Antibody of interest for RNA-binding protein immunoprecipitation (RIP)
- Negative Control Antibody
- Precipitation Carrier (e.g. Pellet Paint™ Co-Precipitant or linear Acrylamide (5mg/mL) if samples are intended for Next Gen Sequence library preparation
- RNase A (10mg/mL)
- Reagents for qRT-PCR Analysis and/or Reagents for RNA-Seq Library Construction
Description
- Generates cross-linked chromatin to allow analysis of a variety of chromatin-associated RNAs
- Flexible, scalable input requirements: Recover RNA from millions of cells or as few as 5,000 cells
- Magnetic protein A/G bead blend and optimized buffer system results in low backgrounds and high signal-to-noise ratios
- Suitable for analysis by RT-qPCR or RIP-seq
- Complete set of reagents and detailed protocol to enable first-time success
Specifications
| Store components at the temperatures indicated on the labels | |
| RNA Binding Protein Immunoprecipitation (RIP), Protein Interaction Assays | |
| 24 Assays | |
| Kit components are stable for 6 months from date of shipment when stored as directed |
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