Invitrogen™ LysoLight™ Deep Red Antibody Labeling Kits

LysoLight™ Deep Red Antibody Labeling Kits offer an efficient method for conjugating antibodies with LysoLight™ Deep Red dye. This process allows for specific and sensitive detection of internalized antibodies within lysosomes using fluorescence microscopy, flow cytometry, or high content imaging platforms.

Catalog Number	Mfr. No.	Description	Quantity
L36001	Invitrogen™ L36001	LysoLight™ Deep Red Antibody Labeling Kit	3 x 100 μg
L36002	Invitrogen™ L36002	LysoLight™ Deep Red Antibody Labeling Kit	3 x 1 mg
L36003	Invitrogen™ L36003	LysoLight™ Deep Red Amine Reactive Dye	3 x 100 μg
L36004	Invitrogen™ L36004	LysoLight™ Deep Red Amine Reactive Dye	500 μg

Description

LysoLight Deep Red Antibody Labeling Kits provide a powerful tool for visualizing the lysosomal localization and catabolic degradation of antibodies and proteins. These kits utilize a unique fluorophore that is only active upon enzymatic cleavage by the lysosomal specific enzyme, Cathepsin B. Outside of the lysosome, LysoLight Deep Red has little to no observable background fluorescence, and only upon localization to the protease rich environment of the Lysosome, does LysoLight become activated and brightly fluoresce.

The labeling protocol provided in the kits involves a simple and rapid procedure, ensuring minimal hands-on time and reliable results. The kits include all necessary reagents, including the LysoLight Deep Red dye, buffers, and purification columns, to facilitate the antibody labeling process.

The LysoLight Deep Red SDP ester contained in these kits readily reacts with lysine residues in antibodies or proteins to yield a covalently attached fluorogenic sensor. The spin columns included in the kits are used for purifying the labeled antibody from excess dye with yields of 70–95%. The kits contain sufficient reagents for three labeling reactions of 100 µg or 1 mg of antibody. The user guide describes a general protocol for using the amine-reactive, SDP ester form of the LysoLight Deep Red dye for labeling either 100 µg or 1 mg of IgG.

Specifications

• Color: Deep Red
• For Use With (Application): Antibody and Protein Labeling
• Green Features: Enhanced stability
• Solubility: DMSO (Dimethylsulfoxide)

• Detection Method: Fluorescence
• For Use With (Equipment): Flow Cytometer, Fluorescence Microscope, High Content Analysis Instrument
• Product Line: LysoLight™
• Excitation/Emission: 650/668 nm

For Research Use Only.