Invitrogen™ X-Gal

X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays.

Catalog Number	Mfr. No.	Quantity
15-520-034	Invitrogen™ 15520034	100 mg
15-520-018	Invitrogen™ 15520018	1 g

Description

X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays. Bacteria containing active β-galactosidase produce blue colonies when grown on plates containing X-Gal. X-Gal is supplied as white crystals and is soluble in dimethylformamide.

Primary applications
• Blue-white screening—detect functional β-galactosidase (reporter gene expression)
• Histochemical detection of β-galactosidase activity in fixed tissues
• X-gal overlay assay

Find the media format that fits your needs
• We offer many ready-to-use media formulations, such as LB Broth (Cat. No. 10855001), Terrific Broth (Cat. No. A1374301) or S.O.C recovery medium (Cat. No. 15544034).
• We also offer IPTG (Cat. No. 15529019) for blue-white screening.

Order Info

Approved for shipment at Room Temperature or on Wet Ice.

Specifications

• Detection Method: Colorimetric

For Research Use Only. Not for use in diagnostic procedures.