Invitrogen™ X-Gal

X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays.

Supplier:  Invitrogen™ 15520018

Catalog No. 15-520-018

Description

X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays. Bacteria containing active β-galactosidase produce blue colonies when grown on plates containing X-Gal. X-Gal is supplied as white crystals and is soluble in dimethylformamide.

Primary applications

• Blue-white screening—detect functional β-galactosidase (reporter gene expression)
• Histochemical detection of β-galactosidase activity in fixed tissues
• X-gal overlay assay

Find the media format that fits your needs

We offer many ready-to-use media formulations, such as LB Broth (10855001), Terrific Broth (A1374301) or S.O.C recovery medium (15544034).

We also offer IPTG (15529019) for blue-white screening.

Explore other media formats for bacterial growth.

Order Info

Approved for shipment at Room Temperature or on Wet Ice.

Specifications

• Chemical Name or Material: 5-bromo-4-chloro-3-indolyl-β-D-galactoside
• Detection Method: Colorimetric
• Format: Bottle
• Product Type: X-Gal Substrate
• Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

• Content And Storage: • X-Gal, powder, 1 g
• Description: X-Gal, Cell-Permeant
• For Use With (Application): Immunoblotting
• Quantity: 1 g
• Form: Powder

For Research Use Only. Not for use in diagnostic procedures.