Invitrogen™ MultiShot™ StripWell TOP10 Chemically Competent E. coli

MultiShot StripWell TOP10 Chemically Competent E. coli cells are ideal for high-efficiency cloning and plasmid propagation, achieving a transformation efficiency of >1 x 10⁸ cfu/μg with control plasmid DNA in StripWell format.

Supplier:  Invitrogen™ C409601

Catalog No. C409601

Description

MultiShot StripWell TOP10 Chemically Competent E. coli cells are ideal for high-efficiency cloning and plasmid propagation, achieving a transformation efficiency of >1 x 10⁸ cfu/μg with control plasmid DNA in StripWell format. These cloning competent cells are packaged in a rack containing 12 strips of 8 tubes to increase productivity for throughput bacterial transformations.

Versatile TOP10 cloning capabilities 
MultiShot StripWell TOP10 Chemically Competent E. coli cells are the laboratory workhorse for chemical transformations as they are compatible with a broad spectrum of DNA types for transformation. They allow stable replication of high-copy number plasmids and are the same competent cells that come with many of our cloning kits.

TOP10 E. coli cells are genetically similar to the DH10B strain and have been reported to be more resilient to stress conditions (osmotic shock and acidic pH stress). These cells carry mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr), allowing cloning of both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes. Similar to other DH strains, TOP10 cells contain recA1 and endA1 mutations that increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

MultiShot StripWell TOP10 Chemically Competent E. coli cells offer:
• Transformation efficiencies of >1 x 10⁸ cfu/μg
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones on plates containing either X-Gal or Bluo-Gal
• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
• recA1 for reduced occurrence of non-specific recombination in cloned DNA

Note: TOP10 cells do not require IPTG to induce expression from the lac promoter.

MultiShot StripWell flexible format
Each StripWell of eight connected tubes is designed with the same single-tube, single-use features as our One Shot format tubes. This format allows all steps of the transformation protocol, up to plating, to take place in the same tube, thereby saving time and reducing chance of contamination. After addition of DNA, MultiShot StripWell competent cells can be transformed by heat-shock at 42°C using a water bath, all in the same tube.

StripWell tubes can be cut to permit transformations in a single tube, eliminating wasted aliquots and avoiding freeze-thaws that can result in reduced transformation efficiency.

Benefits of the MultiShot StripWell format include:
• Increased productivity—suitable for high throughput experimentation
• Maximal flexibility—transform as few as 1 tube or as many as 96 from the StripWell rack
• Familiar convenience—add DNA directly to cells, heat shock, and recover in the same tube

Genotype
F^–mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK λ–rpsL(Str^R) endA1 nupG

Find the strain and format that fits your needs
• Many other E. coli strains and formats of chemically competent and electrocompetent cells are also available to meet specific transformation needs.
• For high-throughput transformation, choose from our collection of MultiShot formatted comp cells.
• We also offer bacterial growth media in various formats.

Order Info

Shipping Condition: Dry Ice.

Specifications

• Product Type: Chemically Competent Cells
• Improves Plasmid Quality: Yes (endA1)
• Transformation Efficiency Level: Medium Efficiency (1 x 10⁸ to 1 x 10⁹ cfu/μg)
• Antibiotic Resistance Bacterial: Yes (Streptomycin)
• Blue/White Screening: Yes (lacZΔM15)
• Plasmid: High Copy Plasmid
• Reduces Recombination: Yes (recA1)
• T1 Phage - Resistant (tonA): No
• Format: 8-well Strips
• Quantity: 1 x 96-tube Rack

• Contains F' Episome: No
• Cloning Methylated DNA: Yes (mcrA)
• Content And Storage: • MultiShot StripWell TOP10 Chemically Competent E. coli (1 x 96-stripwell plate at 50 μL/well)
  Store Competent Cells at –80°C.
  
  • pUC19 DNA (1 x 50 μL vial at 10 pg/μL)
  Store pUC19 DNA at –20°C.
  
  • S.O.C. medium (2 x 15 mL bottles)
  Store S.O.C. Medium at 4°C or room temperature.
  
  
• Cloning Unstable DNA: No
• High-throughput Compatibility: High
• Preparing Unmethylated DNA: No
• Shipping Condition: Dry Ice
• Species: E. coli (K12)
• Product Line: MultiShot™

For Research Use Only. Not for use in diagnostic procedures.