Invitrogen™ ElectroMAX™ DH10B T1 Phage-Resistant Competent Cells

ElectroMAX DH10B T1 Phage-Resistant Competent Cells offer transformation efficiencies that are among the highest we offer and can exceed 1 x 10¹⁰ cfu/μg plasmid DNA in efficiency.

Supplier:  Invitrogen™ 12033015

Catalog No. 12-033-015

Includes: ElectroMAX DH10B T1 Phage-Resistant Cells: 5 vials, 100μL each, pUC19 DNA (10pg/μL): 1 vial, 50μL, S.O.C. medium: 2 bottles, 6mL each

Description

ElectroMAX DH10B T1 Phage-Resistant Competent Cells offer transformation efficiencies that are among the highest we offer and can exceed 1 x 10¹⁰ cfu/μg plasmid DNA in efficiency. They are ideal for applications requiring high-efficiency transformation, including cDNA and gDNA library construction.

ElectroMAX DH10B cells:
• Maximize yield of transformants from limited cloning products
• Permit efficient cloning of methylated DNA
• Are resistant to T1 and T5 bacteriophages
• Support blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
• Deliver high-yield plasmid preparations for downstream applications

Phage-resistant representative libraries from a single transformation
The tonA mutation in DH10B cells confers resistance to bacteriophages T1 and T5, safeguarding valuable libraries against contamination. Additionally, mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) make ElectroMAX DH10B T1 Phage-Resistant Cells ideal for construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA and allow for efficient plasmid rescue from eukaryotic genomes. ElectroMAX DH10B T1 Phage-Resistant Cells are suitable for construction of gene banks, for the generation of cDNA libraries using plasmid-derived vectors, and for situations when DNA is limiting. This strain also has the Φ80lacZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the endA1 mutation makes DH10B an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.

Genotype
F^-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ^-rpsL nupG tonA

Find the strain and format that fits your needs
• DH10B cells are available in both electrocompetent and chemically competent formats.
• ElectroMAX DH10B cells (Cat. No.18290015) are suitable for cDNA or gDNA library construction but are not resistant to T1 and T5 phage infection.
• MegaX DH10B T1^R Electrocomp Cells (Cat. No. C640003) have the highest transformation efficiency (>3 x 10¹⁰ cfu/μg plasmid DNA) and also offer the benefit of T1 and T5 phage resistance.

Order Info

Shipping Condition: Dry Ice

Specifications

• Product Type: Electrocompetent Cells
• Improves Plasmid Quality: Yes (endA1)
• Transformation Efficiency Level: High Efficiency (>1 x 10⁹ cfu/μg)
• Antibiotic Resistance Bacterial: Yes (Streptomycin)
• Blue/White Screening: Yes (lacZΔM15)
• Plasmid: May be used for plasmids >20 kb
• Reduces Recombination: Yes (recA1)
• T1 Phage - Resistant (tonA): Yes
• Format: Tube
• Quantity: 5 x 100 μL

• Contains F' Episome: No
• Cloning Methylated DNA: Yes (mcrA)
• Content And Storage: • ElectroMAX DH10B T1 Phage-Resistant Cells (5 x 100 μL)
  Store Competent Cells at –80°C.
  
  • pUC19 DNA (50 μL at 10 pg/μL)
  Store pUC19 DNA at –20°C.
  
  • S.O.C. Medium (2 x 6 mL)
  Store S.O.C. Medium at 4°C or room temperature.
  
  .
• Cloning Unstable DNA: Not suitable for cloning unstable DNA
• High-throughput Compatibility: Low
• Preparing Unmethylated DNA: No
• Shipping Condition: Dry Ice
• Species: E. coli (K12)
• Product Line: ElectroMAX™

For Research Use Only. Not for use in diagnostic procedures.