Invitrogen™ MAX Efficiency™ DH5α-T1^R Competent Cells

MAX Efficiency DH5α T1^R Phage-Resistant Competent Cells are high efficiency chemically competent cells for generating cDNA libraries and were developed to confer resistance to the lytic bacteriophages T1 and T5.

Supplier:  Invitrogen™ 12034013

Catalog No. 12-034-013

Description

MAX Efficiency DH5α T1^R Phage-Resistant Competent Cells are high efficiency chemically competent cells for generating cDNA libraries and were developed to confer resistance to the lytic bacteriophages T1 and T5. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5α increase insert stability and improve the quality of plasmid DNA prepared from minipreps. These cells are suitable for the construction of gene banks or generation of cDNA libraries using derived vectors.
MAX Efficiency DH5α T1^R Phage-Resistant Competent Cells have been prepared by a patented modification of the procedure of Hanahan and yield >1 x 10⁹ transformants/μg of monomer pUC19 DNA in a 100 μL transformation reaction.

MAX Efficiency DH5α T1^R Phage Resistant Competent Cell features
• Resistance to the lytic bacteriophage T1, one of the most dangerous E. coli phages in high throughput laboratories and genomic centers
• The φ80lacZΔM15 marker that provides α-complementation of the ß-galactosidase gene from pUC or similar vectors and, therefore, can be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal
• Capable of being transformed efficiently with large plasmids and can serve as a host for the M13mp cloning vectors if a lawn of DH5α-FT, DH5αF', DH5αF'IQ, JM101, or JM107 is provided to allow plaque formation

Genotype
F^- φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 λ-thi-1 gyrA96 relA1 tonA

Order Info

Shipping Condition: Dry Ice

Specifications

• Product Type: Chemically Competent Cells
• Improves Plasmid Quality: Yes (endA1)
• Transformation Efficiency Level: High Efficiency (>1 x 10⁹ cfu/μg)
• Antibiotic Resistance Bacterial: No
• Blue/White Screening: Yes (lacZΔM15)
• Preparing Unmethylated DNA: No
• Shipping Condition: Dry Ice
• Species: E. coli (K12)
• Product Line: MAX Efficiency™

• Contains F' Episome: No
• Cloning Methylated DNA: No
• Content And Storage: • MAX Efficiency DH5α-T1^R Compentent Cells (5 x 200 μL)
  Store Competent Cells at –80°C.
  
  • pUC19 DNA (50 μL at 10 pg/μL)
  Store pUC19 DNA at –20°C.
  
  • S.O.C. Medium (2 x 6 mL)
  Store S.O.C. Medium at 4°C or room temperature.
  
  
• Cloning Unstable DNA: Not suitable for cloning unstable DNA
• High-throughput Compatibility: Low
• Reduces Recombination: Yes (recA1)
• T1 Phage - Resistant (tonA): Yes
• Format: Tube
• Quantity: 5 x 200 μL

For Research Use Only. Not for use in diagnostic procedures.