Applied Biosystems™ Power SYBR™ Green RNA-to-C_T™ 1-Step Kit

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

Supplier:  Applied Biosystems™ 4391178

Catalog No. 4391178

Description

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

• Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction.
• Formulated to ensure maximum sensitivity and reliability
• Reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets

Includes:

• AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation
• ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA
• An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals
• RNase inhibitor, to prevent degradation of RNA templates
• A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments

Specifications

• For Use With (Application): Real Time PCR (qPCR)
• Product Type: Real Time PCR SYBR Master Mix
• Product Line: RNA-to-C_T™, SYBR™
• Sample Type: RNA
• Purity: UP (Ultra Pure)
• Detection Method: SYBR
• Polymerase: AmpliTaq Gold DNA Polymerase
• Reaction Speed: Standard
• Format: Tube
• Volume: 1 x 1 mL
• For Use With (Equipment): 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
• Passive Reference Dye: ROX (Pre-mixed)

• Kit Type: Green RNA-to-CT 1-Step Kit
• Sufficient For: 40 Reactions at 50 μL
• Reverse Transcriptase: ArrayScript™ UP
• Primer Probe Compatibility: Unlabeled Oligos
• No. of Reactions: 40
• PCR Method: 1-Step qRT-PCR
• Shipping Condition: Wet Ice
• Concentration: 2X (MasterMix), 125X (RT Enzyme Mix)
• Purity or Quality Grade: UP (Ultra Pure)
• Content And Storage: Sufficient for 40 reactions at 50 µL reaction volume and includes:
  • 1 x 1 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1, and optimized buffer components. Store at 2-8°C after opening.
  • 1 x 20 µL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase and RNase Inhibitor. Store at -15 to -30°C.
  
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
• Label or Dye: SYBR™ Green

For Research Use Only. Not for use in diagnostic procedures.