Invitrogen™ I-Block™ Protein-Based Blocking Reagent

Highly purified Casein-based blocking reagent. Thermo Scientific I-Block Protein-Based Blocking Reagent provides superior blocking compared to both dried milk and BSA.

Supplier:  Invitrogen™ T2015

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Catalog No. T2015

Description

• Biotin-free
• Tested in assays using Tropix substrates and alkaline phosphatase conjugates
• Useful as blocking reagent in membrane-based and immunoassay applications
• Suggested working
• concentration is 0.2% (w/v) for detection of nucleic acids on neutral or positively-charged nylon membranes and for immunoassays and protein detection on membranes (nitrocellulose, PVDF, or neutral nylon)
• Concentration of 3% is recommended for protein detection on positively-charged nylon membrane (such as TropilonPlus™ membrane)

Preparation

Prepared in either Tris- or phosphate-buffered saline buffer with heating (40° to 50°C).

 

Applications

Can be used with Western-Light™, Western-Light Plus™, Western-Star™, ELISA-Light™, Southern-Light™, and Southern-Star™ Detection Systems.

Specifications

• Product Type: Reagent
• Product Line: I-Block™, NovaBright™
• Buffer: Blocking Buffers
• Shipping Condition: Room Temperature

• Quantity: 30 g
• For Use With (Equipment): Western-Light™ Detection System, Western-Star™ Detection System, Southern-Star™ Detection System, ELISA-Light™ Detection System, Southern-Light™ Detection System, Western-Light Plus™ Detection System
• Content And Storage: Store the product at room temperature.

For Research Use Only. Not for use in diagnostic procedures.