Invitrogen™ IL-6 Receptor (Soluble) Human ELISA Kit

For the quantitative detection of human sIL-6R.

Supplier:  Invitrogen™ BMS214TEN

Catalog No. 50-180-19

Includes:

• Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human sIL-6R
• HRP-Conjugate anti-human sIL-6R monoclonal antibody
• Human sIL-6R Standard lyophilized, 5ng/mL upon reconstitution
• Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
• Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
• Substrate Solution (tetramethyl-benzidine)
• Stop Solution (1M Phosphoric acid)
• Blue-Dye
• Green-Dye
• Adhesive Films

Description

The Human Interleukin-6 Receptor (Hu IL-6R) ELISA quantitates Hu IL-6R in human serum, plasma, amniotic fluid, urine or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-6R. Principle of the method The Human IL-6R solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL6R (interleukin-6 receptor subunit alpha) is a part of the receptor for interleukin 6. The IL-6 receptor complex is composed of two membrane glycoproteins: the low affinity receptor and the signaltransducing component. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer.

Specifications

• Accession Number: P08887
• Assay Sensitivity: 0.01 ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CD126,IL-6R-1,IL-6RA,IL6QA,IL6RQ,gp80,IL6R
• Intraassay CV: 1.7%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 2 hr. 10 min.

• Assay Range: 0.08-5.0 ng/mL
• Conjugate: HRP
• Sample Type: Amniotic Fluid, Plasma, Serum, Supernatant, Urine
• For Use With (Application): ELISA
• Gene ID (Entrez): 3570
• Interassay CV: 2.2%
• Kit Contents: Pre-coated 96 well plates, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 10 x 96 Tests
• Sample Volume: Amniotic Fluid, 20 μL; Plasma, 10 μL; Serum, 10 μL; Supernatant, 20 μL; Urine,20 μL
• Synonym: CD126, Membrane glycoprotein 80
• Test Time: 1 hr. 20 min.

For Research Use Only