Invitrogen™ PDGF-BB Human ELISA Kit

For the quantitative detection of human PDGF-BB.

Includes: Aluminium pouch(es) with a Microwell Plate coated with antibody to human PDGF-BB
Biotin-Conjugate anti-human PDGF-BB antibody
Streptavidin-HRP
Human PDGF-BB Standard lyophilized, 4ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Catalog Number	Mfr. No.	Quantity
50-246-659	Invitrogen™ BMS2071	96 Tests
50-246-660	Invitrogen™ BMS2071TEN	10 x 96 Tests

Description

The Human Platelet Derived Growth Factor BB (Hu PDGF-BB) ELISA quantitates Hu PDGF-BB in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PDGF-BB. Principle of the method The Human PDGF-BB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.

Specifications

• Accession Number: P01127
• Assay Sensitivity: 4.6 pg/mL
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IBGC5,PDGF-2,PDGF2,SIS,SSV,c-sis
• Intraassay CV: 4%
• Label or Dye: HRP
• Sample Volume: Plasma, 20 μL; Serum, 20 μL; Supernatant, 20 μL
• Synonym: Platelet derived growth factor BB
• Test Time: 1 hr. 20 min.

• Assay Range: 31.3-2,000 pg/mL
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 5155
• Interassay CV: 8.4%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 3 hr. 30 min.

For Research Use Only