Invitrogen™ IL-8 Human Uncoated ELISA Kit with Plates

Specifically engineered for accurate and precise measurement of IL-8 protein levels from samples including serum, plasma, and cell culture supernatants. This second generation kit has increased sensitivity with a range from 2-250pg/mL.

Catalog Number	Mfr. No.	Quantity
50-246-338	Invitrogen™ 88808622	2 x 96 Tests
50-246-341	Invitrogen™ 88808686	10 x 96 Tests
50-246-339	Invitrogen™ 88808676	20 x 96 Tests

Description

The Human Interleukin 8 (IL-8) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-8. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Human IL-8 Ready-SET-Go! ELISA Set contains the necessary reagents, standards, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA)

Specifications

• Accession Number: P10145
• Assay Sensitivity: 2 pg/mL
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1
• Label or Dye: HRP
• Sample Volume: Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
• Synonym: Interleukin-8, CXCL8, NAP-I
• Test Time: 1 hr. 45 min.

• Assay Range: 2-250 pg/mL
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 3576
• Kit Contents: Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Detection Enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards., 96-well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22 and -86)
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 24 hr. 30 min.

For Research Use Only