Invitrogen™ IL-6 Human Instant ELISA™ Kit

Instant ELISA Kit

Supplier:  Invitrogen™ BMS213INST

Catalog No. 50-180-14

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-6, Biotin-Conjugate (anti-human IL-6 monoclonal antibody), Streptavidin-HRP and Sample Diluent, lyophilized
Aluminum pouch(es) with a human IL-6 Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Sample Diluent (Use when an external predilution of the samples is needed)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Control high, lyophilized
Control low, lyophilized
Adhesive Films

Description

The Human Interleukin 6 (Hu IL-6) ELISA quantitates Hu IL-6 in human serum, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Hu IL-6. Principle of the method The Human IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.

Specifications

• Accession Number: P05231
• Assay Sensitivity: 0.92 pg/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: BSF-2,BSF2,CDF,HGF,HSF,IFN-beta-2,IFNB2,IL-6
• Intraassay CV: 6.2%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: -20°C
• Target: Human
• Total Assay Time: 3 hr. 10 min.

• Assay Range: 3.1-200 pg/mL
• Conjugate: Biotin
• Sample Type: Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 3569
• Interassay CV: 7%
• Kit Contents: Pre-coated 96 well Microplate, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard curve strips, Controls, Wash Buffer Concentrate, Substrate Solution, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
• Quantity: 128 Tests
• Sample Volume: Serum, 50 μL; Supernatant, 50 μL
• Synonym: Interferon-beta 2, B-cell stimulatory factor 2,HGF
• Test Time: 20 min.

For Research Use Only