Invitrogen™ IL-1 beta Human ELISA Kit, High Sensitivity

High Sensitivity Sandwich ELISA Kit

Supplier:  Invitrogen™ BMS224HS

Catalog No. 50-180-70

Includes:

• Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-1beta
• Biotin-Conjugate anti-human IL-1beta monoclonal antibody
• Streptavidin-HRP
• Human IL-1beta Standard lyophilized, 500pg/mL upon reconstitution
• Control, lyophilized
• Sample Diluent
• Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
• Amplification Diluent Concentrate (2x)
• Amplification Reagent I
• Amplification Reagent II
• Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
• Substrate Solution (tetramethyl-benzidine)
• Stop Solution (1M Phosphoric acid)
• Adhesive Films

Description

The Human High Sensitivity Interleukin 1 beta (Hu HS IL-1β) ELISA quantitates Hu HS IL-1β in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HS IL-1β. Principle of the method The Human HS IL-1β solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

Specifications

• Accession Number: P01584
• Assay Sensitivity: 0.05 pg/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IL-1,IL1-BETA,IL1F2
• Intraassay CV: 6.7%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 18 hr.

• Assay Range: 0.16-10.0 pg/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant, Urine
• For Use With (Application): ELISA
• Gene ID (Entrez): 3553
• Interassay CV: 8.1%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, Amplification Diluent Concentrate, Amplification Reagent I, Amplification Reagent II, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL; Urine,50 μL
• Synonym: Catabolin, Interleukin-1 beta, IL1F2
• Test Time: 1 hr. 20 min.

For Research Use Only