Invitrogen™ GM-CSF Human ELISA Kit

For the quantitative detection of human GM-CSF.

Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human GM-CSF
HRP-Conjugate anti-human GM-CSF monoclonal antibody
Human GM-CSF Standard lyophilized, 1ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

Catalog Number	Mfr. No.	Quantity
50-182-30	Invitrogen™ BMS283	96 Tests
50-182-33	Invitrogen™ BMS283TEN	10 x 96 Tests

Description

The Human Granulocyte Macrophage Colony Stimulating Factor (Hu GM-CSF) ELISA quantitates Hu GM-CSF in human serum, plasma, amniotic fluid, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu GM-CSF. Principle of the method The Human GM-CSF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.

Specifications

• Accession Number: P04141
• Assay Sensitivity: 2.9 pg/mL
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: GMCSF
• Intraassay CV: 6%
• Label or Dye: HRP
• Sample Volume: Amniotic Fluid, 50 μL; Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Synonym: Molgramostin, Sargramostim
• Test Time: 1 hr. 20 min.

• Assay Range: 7.8-500 pg/mL
• Sample Type: Amniotic Fluid, Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 1437
• Interassay CV: 4.4%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 3 hr. 10 min.

For Research Use Only