Galectin 1 Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Pepro Tech Inc 500P210100UG

Catalog No. 500P210100

Description

AA Sequence of recombinant protein: ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Galectin-1. Anti-Human Galectin-1-specific antibody was purified by affinity chromatography employing an immobilized Human Galectin-1 matrix. Sandwich ELISA: To detect Human Galectin-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Galectin-1 (500-P210Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Galectin-1. Western Blot: To detect Human Galectin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Galectin-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Galectin-1 belongs to a large family of carbohydrate-binding proteins called lectins. Galectin-1can be either monomeric or homodimeric and is found in a wide variety of cells and tissue types. Galectin-1 can control cell growth, proliferation, induce apoptosis of activated T cells while it can also modulate cytokine secretion or inhibit pro-inflammatory cytokine production. Galectin-1 plays an important role in acute and chronic inflammation.

Specifications

• Antigen: Galectin 1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: LGALS1
• Gene Alias: 14 kDa laminin-binding protein; 14 kDa lectin; AA410090; beta-galactoside binding protein; beta-galactoside-binding lectin; beta-galactoside-binding lectin L-14-I; beta-galactoside-binding protein 14kDa; DKFZp686E23103; GAL1; GAL-1; Galaptin; Galbp; galectin 1; galectin 1; LGALS1; Galectin1; galectin-1; galectin-1-like protein; GBP; HBL; H-Galectin-1; HLBP14; HPL; L14; L-14.5; Lactose-binding lectin 1; Lect14; Lectin galactoside-binding soluble 1; lectin, galactose binding, soluble 1; lectin, galactoside binding soluble 1; lectin, galactoside-binding, soluble, 1; lectin, galactoside-binding, soluble, 1 (galectin 1); Lgals1; LGAS1; OPG-1; pm12; Putative MAPK-activating protein PM12; RL 14.5; similar to galactose-binding lectin; S-Lac lectin 1; unnamed protein product
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3956
• Content And Storage: -20°C

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P09382
• Gene Symbols: LGALS1
• Immunogen: E.coli-derived Recombinant Human Galectin-1
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Form: Lyophilized