Thermo Scientific™ FastDigest NcoI

The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).

Supplier:  Thermo Scientific™ FD0573

Catalog No. FERFD0573

Description

Lambda DNA digested with NcoI, 0.7% agarose, 4 cleavage sites

FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Highlights

• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5-15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Specifications

• Content And Storage: • FastDigest NcoI (20 μL)
  • 10X FastDigest Buffer (1.0 mL)
  • 10X FastDigest Green Buffer (1.0 mL)
  
  Store at –20°C.
  
  
• Enzyme: Nco I
• Sensitive to Heat Inactivation: Yes
• Type IIS RE: No
• Product Type: Restriction Enzyme
• Product Line: FastDigest™
• Inactivation Temperature: 65°C

• Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
• Compatible Buffer: 10X FastDigest Buffer/FastDigest Green Buffer
• Optimal Reaction Temperature: 37°C
• Quantity: 20 μL (20 Reactions)
• Research Category: Traditional Cloning
• Overhang: 5' protruding ends