MilliporeSigma™ ReBlot Plus Kit and Reagents

Blots can be stripped of antibodies in approximately 15 minutes at room temperature

Includes: Mild Antibody Stripping Solution (10X), 50mL; Strong Antibody Stripping Solution (10X), 50mL

Catalog Number	Mfr. No.	Product Type	Quantity	Kit Contents	Content And Storage
2500MI	MilliporeSigma™ 2500	Western Blot Kit	Ea.	50mL Mild Antibody stripping solution 10X, 50mL strong Antibody stripping solution 10X	4°C
2504MI	MilliporeSigma™ 2504	Western Blot Stripping Buffer	50 mL	50mL Strong Antibody stripping solution 10X	2°C to 8°C
2502MI	MilliporeSigma™ 2502	Western Blot Stripping Buffer	50 mL	25mL Mild Antibody stripping solution 10X	2°C to 8°C

Description

Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are electrophoresed on SDS-PAGE and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid-based technologies, which allow reuse of Southern and Northern blots, it has been difficult to reuse Western blots.

• No pungent-smelling β-mercaptoethanol is contained in the Antibody Stripping Solution
• Antibody stripping is done at room temperature—no heating of blots is required
• Blots may be reused in 25 min.

Specifications

• For Use With (Application): Western Blot