Invitrogen™ eBioscience™ Immune Response T Cell Receptor Signaling Kit

Kit is comprised of positive control stimulation reagents, buffers, viability dye, antibodies, and compensation beads needed to stimulate and detect phosphorylation of CD247 (CD3 zeta) on tyrosine 142 (Tyr142) in human T cells by flow cytometry.

Supplier:  Invitrogen™ A53422

Catalog No. A53422

Description

• Kit is comprised of positive control stimulation reagents, buffers, viability dye, antibodies, and compensation beads
• Use to stimulate and detect phosphorylation of CD247 (CD3 zeta) on tyrosine 142 (Tyr142) in human T cells by flow cytometry
• Unique spectral fingerprint is designed to provide the brightest signal for flow cytometry experiments
• Complete kit packaged in single-use vials for ease of use, and to ensure stability performance over time
• Extensive validation and protocol optimization for consistent results

Stimulation, fixation, permeabilization, and staining are performed in 96-well, round-bottom plates

Alternatively, cells may be cultured in bulk and transferred to 12 x 75 mm test tubes

Specifications

• Content And Storage: Antibodies, compensation beads, viability stain, buffers
  Store at 4°C and –20°C.
• For Use With (Equipment): Flow Cytometry
• Regulatory Status: RUO

• For Use With (Application): T Cell Receptor Signaling Analysis and Research
• Quantity: 1 Kit
• Shipping Condition: On Ice