Invitrogen™ eBioscience™ Annexin V Apoptosis Detection Kits

Kits utilize viability dye-conjugated annexin V to label phosphatidylserine (PS), an early marker of apoptosis, on the extracellular membrane.

Supplier:  Invitrogen™ 88800674

Catalog No. 50-927-7

Includes: Cat. No. 88-8006-72:
10X Binding Buffer
Annexin V eFluor™ 450 : 5μL/test, 50 tests Protect from light.
7-AAD Viability Staining Solution : 5μL/test, 100 tests Protect from light.
Cat. No. 88-8006-74:
10X Binding Buffer
Annexin V eFluor™ 450 : 5μL/test, 200 tests Protect from light.
7-AAD Viability Staining Solution : 5μL/test, 2x100 tests Protect from light.

Description

Easily distinguish cells undergoing early and late apoptosis with eBioscience and Annexin V Apoptosis Detection kits for flow cytometry analysis. These kits utilize viability dye-conjugated annexin V to label phosphatidylserine (PS), an early marker of apoptosis, on the extracellular membrane. In late-stage apoptosis, as cell integrity is lost, both annexin V and the viability dye traverse and bind PS on the interior of the plasma membrane.

Annexins are a family of calcium-dependent phospholipid-binding proteins that preferentially bind phosphatidylserine (PS). Under normal physiologic conditions, PS is predominantly located in the inner leaflet of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution across the phospholipid bilayer and is translocated to the extracellular membrane leaflet marking cells as targets of phagocytosis. Once on the outer surface of the membrane, PS can be detected by fluorescently labeled annexin V in a calcium-dependent manner.

In early-stage apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI), 7-AAD, or fixable viability dyes such as eFluor 450. These cells will stain with annexin V but not a viability dye, thus distinguishing cells in early apoptosis. However, in late-stage apoptosis, the cell membrane loses integrity, thereby allowing annexin V to also access PS in the interior of the cell. A viability dye can be used to resolve these late-stage apoptotic and necrotic cells (annexin V, viability dye-positive) from the early-stage apoptotic cells (annexin V positive, viability dye-negative).

Current viability stains available with the eBioscience Annexin V Apoptosis Detection kits include FITC, eFluor 450, allophycocyanin (APC), phycoerythrin (PE), PE-cyanine7, and biotin.

Specifications

• Description: eBioscience Annexin V Apoptosis Detection Kit eFluor 450
• Format: Kit
• Product Type: Apoptosis Detection Kit
• Excitation/Emission: 405, 546/450, 647
• Conjugate: eFluor 450, 7-AAD

• Quantity: 200 Tests
• Kit Contents: • Annexin V Binding Buffer (10X), 1 bottle (100 mL)
  • Annexin V eFluor 450, 1 vial (1 mL)
  • 7-AAD Viability Staining Solution, 2 vials (0.5 mL)
  
  Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits.
• Content And Storage: 2°C to 8°C. Avoid temperature fluctuations.
• Shipping Condition: Wet Ice
• For Use With (Equipment): Fluorescence Microscope, Flow Cytometer