Invitrogen™ CyQUANT™ LDH Cytotoxicity Assay

CyQUANT™ LDH Cytotoxicity Assay Kit is a colorimetric assay that provides a simple and reliable method for determining cellular cytotoxicity.

Supplier:  Invitrogen™ C20300

Catalog No. C20300

Description

CyQUANT™ LDH Cytotoxicity Assay Kit is a colorimetric assay that provides a simple and reliable method for determining cellular cytotoxicity. Lactate dehydrogenase (LDH) is a cytosolic enzyme present in many different cell types that is released into the cell culture medium upon damage to the plasma membrane. The CyQUANT LDH Cytotoxicity Assay Kit provides the reagents to accurately and quantitatively measure this extracellular LDH.

CyQUANT™ LDH Cytotoxicity Assay Kit features include:

• Convenient—add-mix-read assay format for adherent and suspension cells, including 3D cell models
• Accurate—provides a quantitative measurement of LDH release
• Flexible—ideal for high-throughput screening, monitor cytotoxicity from the same sample over time
• Robust—uses stable LDH enzyme activity as a cytotoxic marker

LDH is a cytosolic enzyme present in many different cell types and is a well-established and reliable indicator of cellular toxicity. Damage of the plasma membrane results in a release of LDH into the surrounding cell culture medium. This extracellular LDH can be quantified by a coupled enzymatic reaction in which LDH catalyzes the conversion of lactate to pyruvate via NAD+ reduction to NADH. Diaphorase then uses NADH to reduce a tetrazolium salt (INT) to a red formazan product that can be measured at 490 nm. The level of formazan formation is directly proportional to the amount of LDH released into the medium.

The CyQUANT™ LDH Cytotoxicity Assay Kit provides the reagents needed for the simple, reliable colorimetric quantification of cellular cytotoxicity. The kit can be used with different cell types, including 3D cell models, to measure cytotoxicity mediated by chemical compounds as well as cell-mediated cytotoxicity. Since LDH in the medium is the indicator of cellular cytotoxicity, the assay can be used to monitor cytotoxicity from the same sample over time. To perform the assay, an aliquot of the cell culture medium is transferred to a new plate and the reaction mixture is added. After a 30-minute incubation, the reaction is stopped by adding Stop Solution and absorbance is measured using a microplate reader

Specifications

• Cell Permeability: Cell-impermeant
• Content And Storage: • Substrate Mixture, 1 vial lyophilizate
  • Assay Buffer, 1 x 600 μL
  • Lysis Buffer, 2.5 mL
  • Stop Solution, 12 mL
  • LDH Positive Control, 6 μL
  
  Store at -5 to -30°C, protect from light
  
• Detection Method: Colorimetric
• For Use With (Equipment): Microplate Reader
• Format: 96-well plate
• Quantity: 200 Assays

• Color: Red
• Description: CyQUANT™ LDH Cytotoxicity Assay
• For Use With (Application): LDH Cytotoxicity Assay
• Product Type: LDH Cytotoxicity Assay
• Product Line: CyQUANT™
• Shipping Condition: Dry Ice

For Research Use Only. Not for use in diagnostic procedures.