Invitrogen™ Annexin V Ready Flow Conjugates for Apoptosis Detection

Annexin V conjugate stains enable you to easily determine apoptotic cells via flow cytometry.

Catalog Number	Mfr. No.	Conjugate	Excitation/Emission	Color
R37174	Invitrogen™ R37174	Alexa Fluor 488	495/519	Green
R37175	Invitrogen™ R37175	Alexa Fluor 647	650/665	Far-Red
R37176	Invitrogen™ R37176	APC (Allophycocyanin)	650/660	Red
R37177	Invitrogen™ R37177	Pacific Blue	410/455	Blue

Description

Enjoy the convenience of ready-to-use Annexin V Ready Flow stand-alone conjugate stains, which offer up to 100-fold fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry. There is no need to dilute, weigh, or pipette the conjugate stains; simply add one drop into cells, incubate, and analyze.

Annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, an indicator of intermediate stages of apoptosis. The difference in fluorescence intensity between apoptotic and nonapoptotic cells stained with our fluorescent annexin V conjugates, as measured by flow cytometry, is typically about 100-fold.

The Annexin V Ready Flow conjugates are provided as ready-to-use, direct-to-sample apoptotic cell stains. Simply add one drop from the dropper bottle directly into 1 x 106 cells in 5 mM Ca2+-containing cell culture medium or annexin binding buffer (Cat. Nos. V13246 or BMS500BB), incubate, and analyze. The traditional annexin staining protocol can be used as an alternative method by replacing the addition of 5 μL annexin conjugate with the addition of 1 drop from the Ready Flow dropper bottle.

The Annexin V Alexa Fluor 488 conjugate is excitable at 495 nm with an emission peak at 519 nm.
The Annexin V Alexa Fluor 647 conjugate is excitable at 650 nm with an emission peak at 665 nm.
The Annexin V APC conjugate is excitable at 650 nm with an emission peak at 660 nm.
The Annexin V Pacific Blue conjugate is excitable at 410 nm with an emission peak at 455 nm.
Annexin V staining to detect apoptotic cells can only be done on live cells and tissue. If samples are to be fixed post-staining, there are specific conditions required to achieve transient retention of signal. These include use of an alcohol-free, aldehyde-based fixation method, use of buffers containing Ca2+ and avoidance of surfactants/detergents. For your convenience, we also offer a concentrated annexin-binding buffer that facilitates the binding of annexin V to phosphatidylserine in apoptosis assays.

Specifications

• Kit Contents: 2 dropper bottles, store at room temperature
• Content And Storage: Store at room temperature.
• For Use With (Equipment): Flow Cytometer

• Product Type: Ready Flow Conjugate
• Shipping Condition: Room Temperature