Molecular Probes™ Annexin V Conjugates for Apoptosis Detection

Detect early stages of apoptosis with Annexin V stand-alone Alexa Fluor, APC, Pacific Blue, PE, FITC, and biotin conjugates using flow cytometry.

Catalog Number	Mfr. No.	Conjugate	Excitation/Emission	Flow Cytometer Laser Lines
A13201	Molecular Probes™ A13201	Alexa Fluor 488	495/519	488
A13199	Molecular Probes™ A13199	FITC	494/518	488
A13203	Molecular Probes™ A13203	Alexa Fluor 594	590/617	532
A23202	Molecular Probes™ A23202	Alexa Fluor 350	346/442	UV
A23204	Molecular Probes™ A23204	Alexa Fluor 647	650/665	633-637
A35108	Molecular Probes™ A35108	Alexa Fluor 555	555/565	532, 561
A35110	Molecular Probes™ A35110	APC (Allophycocyanin)	650/660	633-637
A35111	Molecular Probes™ A35111	PE	565/578	488, 532, 561

Description

Achieve quick and reliable detection of early cell apoptosis with Annexin V stand-alone conjugates for apoptosis detection. Annexin V conjugates offer up to 100-fold difference in fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry.

Annexin V has a high affinity for phosphatidylserine (PS), which becomes exposed on the outer leaflet of cells undergoing apoptosis. Because of this affinity, fluorescently labeled annexin V reagents are commonly used in apoptosis research.

Annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, an indicator of intermediate stages of apoptosis. The difference in fluorescence intensity between apoptotic and nonapoptotic cells stained with our fluorescent annexin V conjugates, as measured by flow cytometry, is typically about 100-fold.

In collaboration with Nexins Research BV, we provide the best and brightest annexin V conjugates available, including Alexa Fluor 350, 488, 555, 568, 594, 647, and 680 annexin V conjugates, as well as Annexin V APC, Biotin-X, FITC, Pacific Blue, and PE conjugates. Highly fluorescent annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, one of the earliest indicators of apoptosis.

The Annexin V Pacific Blue conjugate is violet excitable, making it ideal for instruments with a violet laser and for multicolor experiments that include green- or red-fluorescent dyes.

The benefits of our annexin V conjugates include:
• Conjugated to Invitrogen Alexa Fluor and eFluor dyes for brighter signals
• Conjugates for all available lasers
• Available as stand-alone reagents or easy-to-use kits

Annexin V staining to detect apoptotic cells can only be done on live cells and tissue. If samples are to be fixed post-staining, there are specific conditions required to achieve transient retention of signal. These include use of an alcohol-free, aldehyde-based fixation method, use of buffers containing Ca2+ and avoidance of surfactants/detergents. For your convenience, we also offer a concentrated annexin-binding buffer that facilitates the binding of annexin V to phosphatidylserine in apoptosis assays.

Specifications

• Product Type: Annexin V conjugate
• Shipping Condition: Wet Ice
• For Use With (Equipment): Flow Cytometer